Open chunxuan-hs opened 3 months ago
Hello chunxuan!
If you have already obtained the MiXCR license, they explain in their website how to activate it. You can follow this steps: https://mixcr.com/mixcr/getting-started/milm/
Once MiXCR is installed and activated, you should add it into the $PATH variable (using the export command):
export PATH=/path_to_MiXCR_directory:$PATH
More info about how to install MiXCR in your computer if you still have problems: https://mixcr.com/mixcr/getting-started/installation/#manual-installation
Alternatively, I recomend you to use Docker so you do not need to install MiXCR in your computer. Follow this steps:
docker pull milaboratory/mixcr
nextflow run nextflow_main.nf -profile docker
Hope this help! Teresa.
Hi Teresa, many thanks for the reply!
Indeed I am using docker, as shown in the README.md of "TCR_nextflow".
In the mixcr official doc, license is used in the following command (an example):
> docker run --rm \
-e MI_LICENSE="...license-token..." \
-v /path/to/raw/data:/raw:ro \
-v /path/to/put/results:/work \
ghcr.io/milaboratory/mixcr/mixcr:latest \
align -s hs \
/raw/data_R1.fastq.gz \
/raw/data_R2.fastq.gz \
alignments.vdjca
However, I am not sure how to config it with the TCR_nextflow as there is no way to pass `-e MI_LICENSE="...license-token..." to the command
nextflow run nextflow_main.nf -profile docker
Sorry I am new to nextflow and my questions might be very stupid.
Hi developer,
We always run into the issue of "cannot execute binary file", and I think it might be related to mixcr licensce. I have obtained the licensce, how do I provide it to the pipeline? Many thanks!