DAMPLAB / damplab-aquarium

The Aquarium Lab Operating System
http://www.aquarium.bio/
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[Cloning][All RNA-Seq Related Protocols] #13

Open rychen58 opened 4 years ago

rychen58 commented 4 years ago

Summary & Context

Jing has started drafting for all RNA-Seq related protocols. Ask Rita for more info.

Steps

  1. mRNA Enrichment (EnrichRNA.rb)
  2. Part 1 of cDNA Library Prep, Fragmentation (fragmentation.rb)
  3. Part 2 of cDNA Library Prep, cDNA Synthesis (cDNA.rb)
  4. Part 3 of cDNA Library Prep, Adaptor Ligation (ligation.rb)
  5. Part 4 of cDNA Library Prep, PCR Enrichment of Adaptor Ligated DNA (PCR.rb)