Rita has already started a draft for this protocol by modifying Extract Gel Fragment, which is currently broken in Aquarium.
Request for this protocol:
1) A direct linkage protocol for PCR -> Run on gel -> Verify gel fragment -> PCR Purification
i) PCR Purification w/o DpnI Digestion
ii) Create new run on agarose gel protocol (one for gel extraction and the other for gel verification)
Summary & Context
Rita has already started a draft for this protocol by modifying Extract Gel Fragment, which is currently broken in Aquarium. Request for this protocol: 1) A direct linkage protocol for PCR -> Run on gel -> Verify gel fragment -> PCR Purification i) PCR Purification w/o DpnI Digestion ii) Create new run on agarose gel protocol (one for gel extraction and the other for gel verification)
Steps
1. 2. 3. 4.