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DHowett / qlbreader

"qlb reader" :P
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Parse QLR and QLP files #11

Open seaaan opened 7 years ago

seaaan commented 7 years ago

There is one .qlr and one .qlp file per plate (project). They seem to contain a lot of similar information. What is the difference?

They also are likely to contain the summarized droplet data. They definitely contain the data exported in the _Amplitude files, in that the numbers exactly match. They also seem to contain additional numbers per droplet, perhaps they have pulse height, width, and area, whereas the _Amplitude files seem to just be height. The data is already compensated.

Perhaps they also store the extra droplets that are not included in the _Amplitude files. This would help to determine how they are filtering those droplets out. Or maybe they at least appear in the same order that the pulses appear in the raw data. That could help line up the droplets I counted with the droplets they counted and figure out why they leave out what they leave out.

seaaan commented 7 years ago

The .qlr and .qlp files grow in proportion to the number of wells used in the plate:

Wells QlrFileInKb QlpFileInKb File
40 15789 15825 001 aza_2016-07-11-11-03
48 23234 23265 001 aza singles_2016-07-12-15-17
24 11472 11518 009 dna reference_2016-05-19-15-09
48 24817 24848 009 dna reference plate 2_2016-05-26-15-51
16 6754 6805 40 cycles_2016-06-29-14-15 (017 HIV gDNA ddPCR)