I use the following command to assembly my illumina data with nanopore, but there are a warning showing below.
My command:
nohup ~/tools/SPAdes-3.13.0-Linux/bin/metaspades.py -1 ~/data/seameta_illumina/seameta_9_illu/seameta_9/seameta-9_illu_1.fq -2 ~/data/seameta_illumina/seameta_9_illu/seameta_9/seameta-9_illu_2.fq --nanopore ../seameta_9_1kb+.fa -t 70 -o ./ &
=== Error correction and assembling warnings:
4:21:53.059 66G / 163G WARN ScaffoldingUniqueEdgeAna (scaff_supplementary.cpp : 59) Less than half of genome in unique edges!
======= Warnings saved to /home/wzq/data/seawater_meta_9/workspace/hybrid_raw/warnings.log
what is the warning meaning for?
what is the "59) Less than half of genome in unique edges!"? How to explain it?
Not entirely sure you're in the right place here
I'd suggest reposting on biostars or stack exchange or stack overflow
this also may help; https://github.com/ablab/spades/issues/78
I use the following command to assembly my illumina data with nanopore, but there are a warning showing below. My command: nohup ~/tools/SPAdes-3.13.0-Linux/bin/metaspades.py -1 ~/data/seameta_illumina/seameta_9_illu/seameta_9/seameta-9_illu_1.fq -2 ~/data/seameta_illumina/seameta_9_illu/seameta_9/seameta-9_illu_2.fq --nanopore ../seameta_9_1kb+.fa -t 70 -o ./ &
=== Error correction and assembling warnings:
what is the warning meaning for? what is the "59) Less than half of genome in unique edges!"? How to explain it?