I am having this issue with some older PE files, running HISAT2. I did read the older posts.
It appears that the files have a different number of lines.
Yet, I tried tophat2 and it mapped without problem.
I understand I should sync the FASTQ files, yet, I was wondering why tophat2 can map them and HISAT2 complains. Are they not both using bowtie2 ?
Hi,
I am having this issue with some older PE files, running HISAT2. I did read the older posts. It appears that the files have a different number of lines. Yet, I tried tophat2 and it mapped without problem. I understand I should sync the FASTQ files, yet, I was wondering why tophat2 can map them and HISAT2 complains. Are they not both using bowtie2 ?
Thanks