Hi all, I'm trying to align my reads to a referenceusing hisat2. This has gone smoothly multiple times before, but for this set it's not working at all. The current issue is that when I run hisat2, it actually does produce a sam file, but the stats output says
Segmentation fault (core dumped) (ERR): hisat2-align exited with value 139
I'm not sure how to check if the sam file is good + the stats are really what's critical to me at the moment. What's the issue here? How can I fix it?
For some background: while indexing my reference file, it took much longer than usual (4.5 hours or so, compared to <1h as usual). For what it's worth, my reference fasta is 3.8G, and I'm wondering if the size is part of the reason hisat2 isn't working properly?
Hi all, I'm trying to align my reads to a referenceusing hisat2. This has gone smoothly multiple times before, but for this set it's not working at all. The current issue is that when I run hisat2, it actually does produce a sam file, but the stats output says
Segmentation fault (core dumped) (ERR): hisat2-align exited with value 139I'm not sure how to check if the sam file is good + the stats are really what's critical to me at the moment. What's the issue here? How can I fix it?
For some background: while indexing my reference file, it took much longer than usual (4.5 hours or so, compared to <1h as usual). For what it's worth, my reference fasta is 3.8G, and I'm wondering if the size is part of the reason hisat2 isn't working properly?