For the --rna_strandness command, is there an easy way to tell which direction my reads are supposed be? Are there any programs that can help determine this?
I'm using an NEBNext Small RNA kit (similar to Illumina TruSeq Small RNA) and an Illumina NextSeq 500 machine. Both the company making the kit and the company who made the machine have been unclear about this or said they can't help with 3rd-party software. Is there any sort of guide online for how the read direction in those cases?
For the --rna_strandness command, is there an easy way to tell which direction my reads are supposed be? Are there any programs that can help determine this?
I'm using an NEBNext Small RNA kit (similar to Illumina TruSeq Small RNA) and an Illumina NextSeq 500 machine. Both the company making the kit and the company who made the machine have been unclear about this or said they can't help with 3rd-party software. Is there any sort of guide online for how the read direction in those cases?