Open paulinarosales opened 2 weeks ago
Hello,
I would like to ask about the use of --directional-mapping-reverse on HISAT3N, mainly if the spacification of --rna-strandness RF is needed as well, also if those options are equivalent or both need to be added for reverses stranded libraries?
--directional-mapping-reverse
--rna-strandness RF
Thanks!
Hello,
I would like to ask about the use of
--directional-mapping-reverseon HISAT3N, mainly if the spacification of--rna-strandness RFis needed as well, also if those options are equivalent or both need to be added for reverses stranded libraries?Thanks!