search

DaehwanKimLab / tophat

Spliced read mapper for RNA-Seq
http://ccb.jhu.edu/software/tophat
Boost Software License 1.0
91 stars 46 forks source link

Error: Could not find Bowtie index files /project/Ref_Genome/GRCm38.genome.fa.*.ebwt) #27

Open 52teth opened 8 years ago

52teth commented 8 years ago

I have run tophat2 using cmd: tophat2 --bowtie1 -p 6 -G /project/Ref_Genome/GRCm38.gtf -N 2 -r 40 --library-type fr-unstranded -o ./ /project/Ref_Genome/GRCm38.genome.fa /project/rawdata/mouse-1.fq /project/rawdata/mouse-2.fq but the following error occurs: Error: Could not find Bowtie index files /project/RefGenome/GRCm38.genome.fa..ebwt) I have checked that /project/RefGenome/GRCm38.genome.fa..ebwtl is produced.

My software infomation is here: bowtie version 1.1.1 TopHat v2.0.13

Any suggestion? Thanks a lot!