Tophat Fusion Post error

Hi, I want to annotate tophat fusions.out file with gene names. So after running Tophat with --fusion-search option, I got fusions.out file. Now I want to run tophat-fusion-post. I have cretaed the recommneded diretory structure and have installed blast database and have installed blast by blast.dmg for my mac according to blast website.

When I run the following, I am getting error: tophat-fusion-post -p 8 --num-fusion-reads 1 --num-fusion-pairs 2 --num-fusion-both 5 indexes/hg38

I have directory structure: Tophat_DIr --blast --indexes --tophatoutputsample

--accepted_hits.bam
--align_summary.txt
--deletions.bed
--fusions.out
--insertions.out
--junctions.bed
--logs
--prep_reads.info
--unmapped.bam

--ensGene.txt --refGene.txt

Error: Beginning TopHat-Fusion post-processing run (v2.1.0)

[Mon Feb 26 14:32:42 2018] Extracting 23-mer around fusions and mapping them using Bowtie [Mon Feb 26 14:32:46 2018] Filtering fusions Traceback (most recent call last): File "/usr/local/bin/tophat-fusion-post", line 2924, in sys.exit(main()) File "/usr/local/bin/tophat-fusion-post", line 2895, in main filter_fusion(bwt_idx_prefix, params) File "/usr/local/bin/tophat-fusion-post", line 965, in filter_fusion ensGene_list = read_genes("ensGene.txt") File "/usr/local/bin/tophat-fusion-post", line 917, in read_genes num_exons = int(line[7]) ValueError: invalid literal for int() with base 10: 'exonCount'

Can anyone please tell me whats going wrong?

Thanks, Payal

DaehwanKimLab / tophat

Tophat Fusion Post error #52

Error: Beginning TopHat-Fusion post-processing run (v2.1.0)