Open rkretsch opened 1 year ago
In fact some code already prepared and commented out for this purpose
# new_loop = _get_random_barcode(num_bp=0, num5hang=len(loop),loop='').seq
# uid = new_hang+uid[num5polyA:num5polyA+num_bp]+new_loop+uid[num5polyA+num_bp+len(loop):]
Currently, if desired barcode contains a stem-loop a fix sequence is used for the loop. Currently when the program has problems finding a barcode that matches the desired structure it will
So far these two steps have solved our problems, but if there is a case were the loop sequence is interacting heavily with the sequence of interest we could add