Flexiplex barcode count clarification?

DavidsonGroup / flexiplex

The Flexible Demultiplexer

https://davidsongroup.github.io/flexiplex/

MIT License

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Flexiplex barcode count clarification? #37

Closed cnk113 closed 3 months ago

cnk113 commented 3 months ago

Hi,

I'm running flexiplex in discovery mode with UMI and a CB. I was wondering the output flexiplex_barcodes_counts.txt is UMI count or read count per barcode?

Thanks!

nadiadavidson commented 3 months ago

Hi,

They are read counts (so no deduplication using UMIs).

Cheers, Nadia.