Because the sequencing quality is not very good, sometimes a long gene breaks into several unconnected fragments during transcriptome splicing. However, in the process of clustering, the software will often identify these fragments into multiple copies. How to solve this situation?
thank you！
Because the sequencing quality is not very good, sometimes a long gene breaks into several unconnected fragments during transcriptome splicing. However, in the process of clustering, the software will often identify these fragments into multiple copies. How to solve this situation? thank you！