Since mustv2 runs more than 20 days. I terminated the programme and split the genome, whereupon mustv2 issued the following errors.
Spliting the genome into 10 sub-genomes ... [SubFiles:10] [done] [Elapsed time: 0 seconds] Scanning the nucleotide sequences for potential MITEs ... Total calculation time elapsed: 146 seconds. Loading the annotation data ... [RawMITEs:54584] [done] [Time:3 seconds] Removing redundancy in the predicted MITEs ... [RuleBasedRemoving:3] [MITE:54581] [done] [Time:3 seconds] Clustering ... [BLAST] [done] [ClusterNum:1492] [Time:378 seconds] Deciding the strand information of each valid MITE ... [done] [Time:4 seconds] Saving the data ... [done] [ValidMITENum:6526] [Time:12 seconds] Screening for all the MITE copies ... [BackupMITEs:2078] [Templates:2078] [BLAT] [OtherCopy:1496] [AllCopySaved:3574]"/home/ram/transposon_annotation_tools_env/bin/MUST.r2-4-002.Release/refineBoundary.pl" "temp" 0.8 "temp/temp-genome-seq.fasta" "result.txt.be-filter1" "result.txt.be-filter2" -Error when refineBoundary!
Hi Kevin,
We too encounter the same error with "must"....... Error when refineBoundary!
This troubles the downstream processes like parseAnnotation, which i raised at #16.
Please suggest.
Dear Kevin,
Since mustv2 runs more than 20 days. I terminated the programme and split the genome, whereupon mustv2 issued the following errors.
Spliting the genome into 10 sub-genomes ... [SubFiles:10] [done] [Elapsed time: 0 seconds] Scanning the nucleotide sequences for potential MITEs ... Total calculation time elapsed: 146 seconds. Loading the annotation data ... [RawMITEs:54584] [done] [Time:3 seconds] Removing redundancy in the predicted MITEs ... [RuleBasedRemoving:3] [MITE:54581] [done] [Time:3 seconds] Clustering ... [BLAST] [done] [ClusterNum:1492] [Time:378 seconds] Deciding the strand information of each valid MITE ... [done] [Time:4 seconds] Saving the data ... [done] [ValidMITENum:6526] [Time:12 seconds] Screening for all the MITE copies ... [BackupMITEs:2078] [Templates:2078] [BLAT] [OtherCopy:1496] [AllCopySaved:3574]"/home/ram/transposon_annotation_tools_env/bin/MUST.r2-4-002.Release/refineBoundary.pl" "temp" 0.8 "temp/temp-genome-seq.fasta" "result.txt.be-filter1" "result.txt.be-filter2" -Error when refineBoundary!
while using demo file, there is no error.
Please send me your suggestion.
with regards