I am new to use kraken2. I had made a serial parameters that has a nice result with read len 75 (such as confidence).
But when I try to use read len 100, I got a lot of false positive, for example taxid 28112： (28112:66;) .
Then I blast the sequence (28112:66), I got high bit score another correct taxid.
I wonder why this will happen that taxid 28112 get a high confidence, but it's a FP, that use blast can get a high bitscore、high identity another and uniq taxid？
Any help will be appreciated! Thank you !
I am new to use kraken2. I had made a serial parameters that has a nice result with read len 75 (such as confidence). But when I try to use read len 100, I got a lot of false positive, for example taxid 28112： (28112:66;) . Then I blast the sequence (28112:66), I got high bit score another correct taxid. I wonder why this will happen that taxid 28112 get a high confidence, but it's a FP, that use blast can get a high bitscore、high identity another and uniq taxid？ Any help will be appreciated! Thank you !