Open pratibha-kadam opened 4 months ago
Please provide what the screen output is
Please see below the command that I ran: kraken2 --db /media/fmr/HDD1/kdb --threads 15 --classified-out --paired --gzip-compressed /media/fmr/HDD1/Wastewater_AMR_Analysis_2024_07_27/3.Cutadapt/MT_2024_0708/Trimmed_MT_2024_0708_R1.fastq.gz /media/fmr/HDD1/Wastewater_AMR_Analysis_2024_06_27/3.Cutadapt/MT_2024_0708/Trimmed_MT_2024_0708_R2.fastq.gz --output ./MT_2024_0708_out.kraken --memory-mapping --report ./MT_2024_0708_report
please see the attached output
That's all !!
I have used Kraken2 before on the same 64 GB RAM computer with 16 threads. The drive where the data and output directory are present has more than 300GB of space. I was trying to Kraken2 classification step for paired end data. In my database, I have only 3 k2d database files. When I run the command every time it takes 4-5 hours to load the database information and when it processes the file it suddenly disappears after a while.
I tried it a couple of times but it vanishes every time.
Please let me know where I go wrong or any system issue.