Parameters for database construction and classification with Nanopore v14 chemistry

DerrickWood / kraken2

The second version of the Kraken taxonomic sequence classification system

MIT License

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Parameters for database construction and classification with Nanopore v14 chemistry #874

Open rjsorr opened 3 months ago

rjsorr commented 3 months ago

Hi,

can you please provide some guidelines for building of database and classifaction of reads (parameters) for nanopore v14 chemistry (10.4 flow cells). Read quality and length surely have an impact on choices and results? The new chemistry is now averaging >q20 and more are starting to adopt it.