Dfam-consortium / RepeatModeler

De-Novo Repeat Discovery Tool
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error in builddatabase #238

Open weirdo-onlooker opened 3 months ago

weirdo-onlooker commented 3 months ago

During the database construction process, "BuildDatabase -name hhh_YQtrio0 -engine ncbi genome/hhh_YQtrio0.fasta" , I also tried to remove the engine option, finally I received the following message:

Building database hhh_YQtrio0: Reading genome/hhh_YQtrio0.fasta... The makeblastdb program exited with code 1. Please check your input file(s) for potential formating errors. /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb returned: /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb: /lib64/libm.so.6: version GLIBC_2.29' not found (required by /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb) /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb: /lib64/libc.so.6: versionGLIBC_2.33' not found (required by /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb) /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb: /lib64/libc.so.6: version GLIBC_2.32' not found (required by /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb) /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb: /lib64/libc.so.6: versionGLIBC_2.34' not found (required by /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb)

The command used was: /ifs1/User/lengliang/yaozc/biosoft/rmblast-2.14.1/bin/makeblastdb -blastdb_version 4 -out hhh_YQtrio0 -parse_seqids -dbtype nucl -in ./RM_9ImDCpo 2>&1 Died at /ifs1/User/lengliang/yaozc/biosoft/RepeatModeler-2.0.5/BuildDatabase line 331.

I have checked my fasta file and there seems to be no issue with it. I attempted to upgrade the GLIBC version, but failed and do not have root permissions. I also tried to downgrade the rmblast version to 2.11.0, but it requires reconfiguration, which also encountered issues. Now I want to replace rmblast with another software, and I don't want to rerun perl ./configure. Where can I modify this configuration file?

this is my fasta file(part):

h1tg000001l CTGAGTTTTCATGTATAATGATGAATTAGGCTAAGCATATGTTGCTTTATGTATGTAAATCTGTTTGTATGGTTGATTGTTTGTACTAAATTCACATTGTGATGCAGTTGCATTTCATATTATAATGCAGTAGGTTTTTATTTTAACTGTGATGCAGTAGGTTGTTTGT......

lifan18 commented 3 months ago

Hi,

I also had this problem when I used rmblast-2.14.1. I fixed the issue "GLIBC_2.29 not found" by replacing the rmblast-2.14.1 with rmblast-2.14.0.

Have a try!

Good luck!

Fan