search

DillonHammill / CytoExploreR

Interactive Cytometry Data Analysis
60 stars 13 forks source link

Broken histograms? #154

Closed th-of closed 2 years ago

th-of commented 2 years ago

With my data I often get weird/broken histograms, anyone know what's causing this? Some examples below. FCS 3.1 files exported from a MACSQuant Analyzer flow cytometer.

I load the files with gs <- cyto_setup("FlowFiles", emptyValue=FALSE, gatingTemplate = "Manual-Gating.csv")

I do a logicle transformation gs <- cyto_transform(gs, type = "logicle")

Then plot figure <- cyto_plot(newgs, parent = NULL, channels = "FL2-A", ... , ... , ... )

Untitled picture 2 Untitled picture Untitled
DillonHammill commented 2 years ago

@th-of this is a known bug in CytoExploreR which has been fixed in the soon to be released version 2.0.0. I would recommend updating when it becomes available. In the meantime you may need to use ggcyto to plot your histograms.