I am getting this error when computing spillover with both cytoExploreRData data and my own data. I'm not sure why. The plotting, gating, and generated % seems to work, but it fails at the last step.
> spill <- cyto_spillover_compute(gs,
+ parent = "Single Cells",
+ spillover = "Spillover-Matrix.csv")
Select a fluorescent channel for the following sample:
Compensation Controls_APC-Cy7 Stained Control.fcs
Compensation Controls_APC Stained Control.fcs
Compensation Controls_FITC Stained Control.fcs
Compensation Controls_Pacific Blue Stained Control.fcs
Compensation Controls_PE-Cy7 Stained Control.fcs
Compensation Controls_PE Stained Control.fcs
Compensation Controls_PerCP-Cy5-5 Stained Control.fcs
Select the lower and upper bounds of the APC-Cy7-A- population to construct an interval gate.
Select the lower and upper bounds of the APC-Cy7-A+ population to construct an interval gate.
Select the lower and upper bounds of the APC-A- population to construct an interval gate.
Select the lower and upper bounds of the APC-A+ population to construct an interval gate.
Select the lower and upper bounds of the FITC-A- population to construct an interval gate.
Select the lower and upper bounds of the FITC-A+ population to construct an interval gate.
Select the lower and upper bounds of the Pacific Blue-A- population to construct an interval gate.
Select the lower and upper bounds of the Pacific Blue-A+ population to construct an interval gate.
Select the lower and upper bounds of the PE-Cy7-A- population to construct an interval gate.
Select the lower and upper bounds of the PE-Cy7-A+ population to construct an interval gate.
Select the lower and upper bounds of the PE-A- population to construct an interval gate.
Select the lower and upper bounds of the PE-A+ population to construct an interval gate.
Select the lower and upper bounds of the PerCP-Cy5-5-A- population to construct an interval gate.
Select the lower and upper bounds of the PerCP-Cy5-5-A+ population to construct an interval gate.
Error in unlink(tmp, recursive = TRUE) :
3 arguments passed to .Internal(unlink) which requires 4
I am getting this error when computing spillover with both cytoExploreRData data and my own data. I'm not sure why. The plotting, gating, and generated % seems to work, but it fails at the last step.