Open kim619 opened 5 months ago
I am not an expert so take my comment with a grain of salt. When I have gotten this message, it often seems that I have set the gain on my flow channel a little too high so that my unstained cells are above the first decade.
Empirically, I have ended up setting m=6 or m=7 or going with an arcsinh transform in these circumstances to make the error message go away.
Here is where to read a little more on the theory of the transform: Cytometry A. 2012 Apr; 81(4): 273–277. Published online 2012 Mar 12. doi: [10.1002/cyto.a.22030]
Hi I am trying to transform my data using the cyto_transform function but it encounters into error possibly due to the parameters used in cyto_transform (e.g, w, m)
Here are the codes
I then tried using different parameters:
I also tried using biexponential transformation:
Same issues persist, please let me know what do the parameters mean since I don't know their meaning it would be hard to tweak around or what can I do about this, thanks a lot!