Hi I am trying to transform my data using the cyto_transform function but it encounters into error possibly due to the parameters used in cyto_transform (e.g, w, m)
Here are the codes
gs<- cyto_transform(gs,channels=c("FITC-H","PE-H","FITC-A","PE-A","FSC-H","SSC-H","FSC-A","SSC-A","Time"),
display=10000,
trans=NULL,
type="log",
select="pCon08-G4.fcs",
m=5,
w=0.5,
widthBasis=80,
axes_limits="data")
Error: 'trans' must be a list of transformer objects (generated by scales::trans_new method)
I then tried using different parameters:
trans_logicle <- cyto_transformer_logicle(gs)
Error in .lgclTrans(x, p, ...) : w is negative!Try to increase 'm'
trans_logicle <- cyto_transformer_logicle(gs,
m=10,
w=-1000)
Error: 'trans' must be a list of transformer objects (generated by scales::trans_new method)
trans_logicle <- cyto_transformer_logicle(gs,
m=10,
w=1)
Error: 'trans' must be a list of transformer objects (generated by scales::trans_new method)
trans_logicle <- cyto_transformer_logicle(gs,
channels = "PE-A",
m=20,
w=10)
Error: 'trans' must be a list of transformer objects (generated by scales::trans_new method)
I also tried using biexponential transformation:
# Default biexponentail transformer
trans_biex <- cyto_transformer_biex(gs)
Error: 'trans' must be a list of transformer objects (generated by scales::trans_new method)
Same issues persist, please let me know what do the parameters mean since I don't know their meaning it would be hard to tweak around or what can I do about this, thanks a lot!
Hi I am trying to transform my data using the cyto_transform function but it encounters into error possibly due to the parameters used in cyto_transform (e.g, w, m)
Here are the codes
I then tried using different parameters:
I also tried using biexponential transformation:
Same issues persist, please let me know what do the parameters mean since I don't know their meaning it would be hard to tweak around or what can I do about this, thanks a lot!