Have problems with PE_merging

[pvannucchi]

Hello, I'm new to the program and I'm trying to analyze some fastq files I jusr received. Reads are already demultiplexed, so I put them in the 2_Demultiplexing folder. Whenever I try to merge PE reads I get this error message: 09:32:42: Finding all file pairs in your input. This may take a while. 09:32:42: Found 1 matching file pairs in 2 input files. 09:32:42: Starting to merge forward and reverse reads. joblib.externals.loky.process_executor._RemoteTraceback: """ Traceback (most recent call last): File "c:\users\pvannucchi\appdata\local\programs\python\python39\lib\site-packages\joblib\externals\loky\process_executor.py", line 463, in _process_worker r = call_item() File "c:\users\pvannucchi\appdata\local\programs\python\python39\lib\site-packages\joblib\externals\loky\process_executor.py", line 291, in call return self.fn(*self.args, self.kwargs) File "c:\users\pvannucchi\appdata\local\programs\python\python39\lib\site-packages\joblib\parallel.py", line 589, in call return [func(*args, *kwargs) File "c:\users\pvannucchi\appdata\local\programs\python\python39\lib\site-packages\joblib\parallel.py", line 589, in return [func(args, kwargs) File "c:\users\pvannucchi\appdata\local\programs\python\python39\lib\site-packages\apscale\b_pe_merging.py", line 73, in pe_merge re.findall("(\d+) Pairs", content)[0], IndexError: list index out of range

Could you please indicate me how I could fix this problem? Thank you in advance.

[DominikBuchner]

Can you please check your vsearch version?

[pvannucchi]

Hi Dominik,

I was able to fix the problem (I just had to copy de dll file into the new vsearch folder again) and now with the downgraded version of Vsearch, it works!

Thank you so much for your help.

Cheers,

Patrizia Elena Vannucchi Área Ecología Acuática Eurofins Cavendish, S.L.U. Ctra. Bailén-Motril, parc. 102 B, Edif. Cristal 2, Pol. Juncaril C.P.: 18210 – Peligros (Granada) Spain Phone: +34 663701104

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De: DominikBuchner @.> Enviado el: domingo, 17 de septiembre de 2023 21:29 Para: DominikBuchner/apscale @.> CC: Patrizia Elena Vannucchi @.>; Author @.> Asunto: Re: [DominikBuchner/apscale] Have problems with PE_merging (Issue #9)

Can you please check your vsearch version?

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