Closed shenghuanjie closed 3 years ago
@westonstauffer. Please give a thumb up if it is working for you.
ROIs in manager now have cell numbers but still have duplicates. For example:
Image 1 cell 1
Image 1 cell 2 is a duplicate of Image 1 cell 1
Image 1 cell 3 is the true cell 2 in this example
Cell filters other than the pre-watershed filter identify additional incorrect cells for analysis as in this example:
Without cell filters. Yellow outline shows selected cells
With pre-watershed filter of 500-Infinity, two cells with >500 pixels are selected and shown in analysis table
With cell filter 1 set to "Area" and 500-Infinity there are additional cells shown with yellow outline, and additional cells in data table
ROIs in manager now have cell numbers but still have duplicates. For example:
The following change is just a disaster waiting to happen.
ImageJ should not have decreased the visibility of part of a protected function.
What happened recently is the update to RoiManager
got separated into a private function from the protected saveResults
we override in EzColocalization
.
After this change, there is no way for us to have control over the update happening in RoiManager since the new addToRoiManager
is a private method.
https://github.com/imagej/imagej1/blob/ba4a8ee10f3cbe06393f4046a88bfa67683aedfd/ij/plugin/filter/ParticleAnalyzer.java#L910-L912
Heat maps scaled by "image" and "stack" do not clear outside of ROIs and are identical.
Are you sure we were clearing the outside of ROIs before for "image" and "stack"?
Heat maps scaled by "image" and "stack" do not clear outside of ROIs and are identical.
Are you sure we were clearing the outside of ROIs before for "image" and "stack"?
You are right. We didn't clear outside of ROIs in "image" or "stack" scaling. I have found that the "stack" scaling is scaling by the first image in the stack. In this example, Image 1 in a stack has 1/5 its normal intensity and for "cell" and "stack" scaling looks similar:
"image" scaling
"stack" scaling
But other images are shifted to mostly maximum values for the "stack" scaling because image 1 had such a lower range for scaling
"stack" scaling
"image" scaling
Fixes #8 Fixes #9
Change file from CRLF to LF Remove unused imports and reorganize imports