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DrHanLim / EzColocalization

GNU General Public License v3.0
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Time-lapse incompatibility with ROIs and ROI errors #6

Closed westonstauffer closed 5 years ago

westonstauffer commented 5 years ago

We have found that time-lapse images stored in a series of frames, instead of a z-stack are not compatible with analysis when an ROI manager is used as cell identification. Trying to perform analysis with this combination leads to an error or inability of the plugin to run. An immediate workaround is to convert a series of "frames" into a series of "slices" (a z-stack), but an update will be released shortly which fixes this incompatibility. To convert frames to slices, open a time series of images, and then select "Image"->"Stacks"->"Stacks to Images" from the menu bar. Next, select "Image"->"Stacks"->"Images to Stacks", and save the produced stack. Repeat this for every channel to be analyzed and the time series of images can now be used with ROIs.

In a separate issue, if any slice in a z-stack has no ROIs for analysis and the ROI manager is used for cell selection, non-critical errors are reported, as no analysis can be performed for that slice. This is a non-critical error in that analysis will run correctly for other images in the stack. These errors will be preceded by the warning: "No cell is found based on the selected filters in some slices." within the error window in a new EzColocalization version released in the near future.

shenghuanjie commented 5 years ago

This bug is fixed in 1.1.3.