EBI-Metagenomics / EukCC

Tool to estimate genome quality of microbial eukaryotes
GNU General Public License v3.0
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Folder mode missed results for some bins #43

Open zhenjiaofenjie opened 1 year ago

zhenjiaofenjie commented 1 year ago

Hi,

I have several bins (say 1.faa 2.faa ... 100.faa) in a bin/ folder. In single mode, EukCC successfully gave results for ~ 70% of them. However, when in folder mode, some of the bins were lost from the eukcc.csv file. Is there a reason for that?

Also, the folder mode merged two bins together, even though I did not provide the link table. Would this be a bug or an expected behavior?

Thanks! Jing

rrohwer commented 1 month ago

Hi, I am having this same issue. I ran eukcc on 73 bins, but only 6 appear in the output file. However, in the log I can see that all bins were recognized, and several even had fairly high completeness but were not reported.

This seems like a bug to me.

Thanks, Robin

# the command I used:
eukcc folder --out eukcc_output --threads 20  --db ../eukccdb/eukcc2_db_ver_1.1 --suffix .fna folder_with_73_MAGs

# output file has practically nothing in it
cat eukcc.csv
bin     completeness    contamination
bin102.fna     0.0     0.0
bin92.fna        7.32    0.0
bin96.fna        0.0     0.0
bin261.fna       10.6    1.0
bin115.fna       1.4     0.0
bin57.fna        3.17    0.0

# but in the log you can see that all 73 bins were found, and some of them were unreported even though they had decent completeness
cat eukcc.log
29-09-2024 14:46:29:  EukCC version 2.1.0
29-09-2024 14:46:29:  Found 73 bins
29-09-2024 14:56:30:  Searching for marker genes in base database
29-09-2024 14:56:31:  No placement marker genes found.
29-09-2024 14:56:31:  Searching for marker genes in base database
29-09-2024 14:56:31:  No placement marker genes found.
29-09-2024 14:56:31:  Searching for marker genes in base database
29-09-2024 14:56:31:  No placement marker genes found.
29-09-2024 14:56:31:  Searching for marker genes in base database
29-09-2024 14:56:31:  No placement marker genes found.
29-09-2024 14:56:32:  Searching for marker genes in base database
29-09-2024 14:56:33:  Found 7 marker genes, placing them in the tree using epa-ng
29-09-2024 14:56:46:  Genome belongs to clade: protozoa (Best TaxID: 33846)
29-09-2024 14:56:46:  Searching for marker genes in protozoa database
29-09-2024 14:56:47:  Found 4 marker genes, placing them in the tree using epa-ng
29-09-2024 14:56:50:  Automatically locating best SCMG set
29-09-2024 14:57:17:  ❗The choosen marker gene set is supported by only half (1/4) of the alignments. This generally is an unstable estimate.
29-09-2024 14:57:20:  Searching fasta for selected markers
29-09-2024 14:57:29:  Completeness: 32.74
29-09-2024 14:57:29:  Contamination: 3.54
29-09-2024 14:57:29:  Max silent contamination: 100.0
29-09-2024 14:57:29:  Searching for marker genes in base database
29-09-2024 14:57:29:  No placement marker genes found.
29-09-2024 14:57:29:  Searching for marker genes in base database
29-09-2024 14:57:29:  No placement marker genes found.
29-09-2024 14:57:29:  Searching for marker genes in base database
29-09-2024 14:57:31:  Found 15 marker genes, placing them in the tree using epa-ng
29-09-2024 14:58:04:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 14:58:04:  Searching for marker genes in protozoa database
29-09-2024 14:58:06:  Found 22 marker genes, placing them in the tree using epa-ng
29-09-2024 14:58:30:  Automatically locating best SCMG set
29-09-2024 14:59:02:  ❗The choosen marker gene set is supported by only half (9/22) of the alignments. This generally is an unstable estimate.
29-09-2024 14:59:02:  Searching fasta for selected markers
29-09-2024 14:59:05:  Completeness: 41.18
29-09-2024 14:59:05:  Contamination: 11.76
29-09-2024 14:59:05:  Max silent contamination: 100.0
29-09-2024 14:59:05:  Searching for marker genes in base database
29-09-2024 14:59:06:  No placement marker genes found.
29-09-2024 14:59:06:  Searching for marker genes in base database
29-09-2024 14:59:06:  Found 8 marker genes, placing them in the tree using epa-ng
29-09-2024 14:59:17:  Genome belongs to clade: protozoa (Best TaxID: 6020)
29-09-2024 14:59:17:  Searching for marker genes in protozoa database
29-09-2024 14:59:18:  Found 6 marker genes, placing them in the tree using epa-ng
29-09-2024 14:59:21:  Automatically locating best SCMG set
29-09-2024 14:59:42:  ❗The choosen marker gene set is supported by only half (2/6) of the alignments. This generally is an unstable estimate.
29-09-2024 14:59:43:  Searching fasta for selected markers
29-09-2024 14:59:44:  Completeness: 31.17
29-09-2024 14:59:44:  Contamination: 0.0
29-09-2024 14:59:44:  Max silent contamination: 100.0
29-09-2024 14:59:44:  Searching for marker genes in base database
29-09-2024 14:59:45:  No placement marker genes found.
29-09-2024 14:59:45:  Searching for marker genes in base database
29-09-2024 14:59:45:  No placement marker genes found.
29-09-2024 14:59:45:  Searching for marker genes in base database
29-09-2024 14:59:46:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 14:59:50:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 14:59:50:  Searching for marker genes in protozoa database
29-09-2024 14:59:50:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 14:59:53:  Automatically locating best SCMG set
29-09-2024 15:00:21:  Searching fasta for selected markers
29-09-2024 15:00:21:  Completeness: 0.0
29-09-2024 15:00:21:  Contamination: 0.0
29-09-2024 15:00:21:  Max silent contamination: 100.0
29-09-2024 15:00:21:  Searching for marker genes in base database
29-09-2024 15:00:22:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:00:26:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:00:26:  Searching for marker genes in protozoa database
29-09-2024 15:00:27:  No placement marker genes found.
29-09-2024 15:00:27:  Searching for marker genes in base database
29-09-2024 15:00:27:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:00:30:  Genome belongs to clade: protozoa (Best TaxID: 174815)
29-09-2024 15:00:30:  Searching for marker genes in protozoa database
29-09-2024 15:00:31:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:00:33:  Automatically locating best SCMG set
29-09-2024 15:00:56:  Searching fasta for selected markers
29-09-2024 15:00:56:  Completeness: 7.32
29-09-2024 15:00:56:  Contamination: 0.0
29-09-2024 15:00:56:  Max silent contamination: 100.0
29-09-2024 15:00:56:  Searching for marker genes in base database
29-09-2024 15:00:56:  No placement marker genes found.
29-09-2024 15:00:56:  Searching for marker genes in base database
29-09-2024 15:00:57:  No placement marker genes found.
29-09-2024 15:00:57:  Searching for marker genes in base database
29-09-2024 15:00:57:  Found 3 marker genes, placing them in the tree using epa-ng
29-09-2024 15:01:02:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:01:02:  Searching for marker genes in protozoa database
29-09-2024 15:01:02:  No placement marker genes found.
29-09-2024 15:01:02:  Searching for marker genes in base database
29-09-2024 15:01:03:  No placement marker genes found.
29-09-2024 15:01:03:  Searching for marker genes in base database
29-09-2024 15:01:03:  No placement marker genes found.
29-09-2024 15:01:03:  Searching for marker genes in base database
29-09-2024 15:01:03:  Found 10 marker genes, placing them in the tree using epa-ng
29-09-2024 15:01:16:  Genome belongs to clade: protozoa (Best TaxID: 5878)
29-09-2024 15:01:16:  Searching for marker genes in protozoa database
29-09-2024 15:01:17:  Found 6 marker genes, placing them in the tree using epa-ng
29-09-2024 15:01:21:  Automatically locating best SCMG set
29-09-2024 15:01:42:  ❗The choosen marker gene set is supported by only half (2/6) of the alignments. This generally is an unstable estimate.
29-09-2024 15:01:43:  Searching fasta for selected markers
29-09-2024 15:01:45:  Completeness: 31.17
29-09-2024 15:01:45:  Contamination: 6.49
29-09-2024 15:01:45:  Max silent contamination: 100.0
29-09-2024 15:01:45:  Searching for marker genes in base database
29-09-2024 15:01:45:  No placement marker genes found.
29-09-2024 15:01:45:  Searching for marker genes in base database
29-09-2024 15:01:45:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:01:49:  Genome belongs to clade: protozoa (Best TaxID: 5654)
29-09-2024 15:01:49:  Searching for marker genes in protozoa database
29-09-2024 15:01:50:  No placement marker genes found.
29-09-2024 15:01:50:  Searching for marker genes in base database
29-09-2024 15:01:50:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:01:53:  Genome belongs to clade: protozoa (Best TaxID: 2699528)
29-09-2024 15:01:53:  Searching for marker genes in protozoa database
29-09-2024 15:01:53:  No placement marker genes found.
29-09-2024 15:01:53:  Searching for marker genes in base database
29-09-2024 15:01:54:  No placement marker genes found.
29-09-2024 15:01:54:  Searching for marker genes in base database
29-09-2024 15:01:54:  No placement marker genes found.
29-09-2024 15:01:54:  Searching for marker genes in base database
29-09-2024 15:01:54:  No placement marker genes found.
29-09-2024 15:01:54:  Searching for marker genes in base database
29-09-2024 15:01:55:  No placement marker genes found.
29-09-2024 15:01:55:  Searching for marker genes in base database
29-09-2024 15:01:55:  No placement marker genes found.
29-09-2024 15:01:55:  Searching for marker genes in base database
29-09-2024 15:01:55:  No placement marker genes found.
29-09-2024 15:01:55:  Searching for marker genes in base database
29-09-2024 15:01:55:  No placement marker genes found.
29-09-2024 15:01:55:  Searching for marker genes in base database
29-09-2024 15:01:56:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:02:00:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:02:00:  Searching for marker genes in protozoa database
29-09-2024 15:02:01:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:02:04:  Automatically locating best SCMG set
29-09-2024 15:02:31:  Searching fasta for selected markers
29-09-2024 15:02:32:  Completeness: 0.0
29-09-2024 15:02:32:  Contamination: 0.0
29-09-2024 15:02:32:  Max silent contamination: 100.0
29-09-2024 15:02:32:  Searching for marker genes in base database
29-09-2024 15:02:32:  No placement marker genes found.
29-09-2024 15:02:32:  Searching for marker genes in base database
29-09-2024 15:02:32:  No placement marker genes found.
29-09-2024 15:02:32:  Searching for marker genes in base database
29-09-2024 15:02:33:  Found 3 marker genes, placing them in the tree using epa-ng
29-09-2024 15:02:37:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:02:37:  Searching for marker genes in protozoa database
29-09-2024 15:02:38:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:02:41:  Automatically locating best SCMG set
29-09-2024 15:03:17:  Searching fasta for selected markers
29-09-2024 15:03:25:  Completeness: 10.6
29-09-2024 15:03:25:  Contamination: 1.0
29-09-2024 15:03:25:  Max silent contamination: 100.0
29-09-2024 15:03:25:  Searching for marker genes in base database
29-09-2024 15:03:26:  Found 4 marker genes, placing them in the tree using epa-ng
29-09-2024 15:03:32:  Genome belongs to clade: protozoa (Best TaxID: 33634)
29-09-2024 15:03:32:  Searching for marker genes in protozoa database
29-09-2024 15:03:32:  Found 5 marker genes, placing them in the tree using epa-ng
29-09-2024 15:03:35:  Automatically locating best SCMG set
29-09-2024 15:04:02:  ❗The choosen marker gene set is supported by only half (1/5) of the alignments. This generally is an unstable estimate.
29-09-2024 15:04:06:  Searching fasta for selected markers
29-09-2024 15:04:09:  Completeness: 25.58
29-09-2024 15:04:09:  Contamination: 10.23
29-09-2024 15:04:09:  Max silent contamination: 100.0
29-09-2024 15:04:09:  Searching for marker genes in base database
29-09-2024 15:04:09:  No placement marker genes found.
29-09-2024 15:04:09:  Searching for marker genes in base database
29-09-2024 15:04:10:  No placement marker genes found.
29-09-2024 15:04:10:  Searching for marker genes in base database
29-09-2024 15:04:10:  No placement marker genes found.
29-09-2024 15:04:10:  Searching for marker genes in base database
29-09-2024 15:04:10:  No placement marker genes found.
29-09-2024 15:04:10:  Searching for marker genes in base database
29-09-2024 15:04:11:  Found 11 marker genes, placing them in the tree using epa-ng
29-09-2024 15:04:33:  Genome belongs to clade: protozoa (Best TaxID: 33846)
29-09-2024 15:04:33:  Searching for marker genes in protozoa database
29-09-2024 15:04:34:  Found 8 marker genes, placing them in the tree using epa-ng
29-09-2024 15:04:42:  Automatically locating best SCMG set
29-09-2024 15:05:08:  ❗The choosen marker gene set is supported by only half (1/8) of the alignments. This generally is an unstable estimate.
29-09-2024 15:05:12:  Searching fasta for selected markers
29-09-2024 15:05:20:  Completeness: 29.2
29-09-2024 15:05:20:  Contamination: 0.88
29-09-2024 15:05:20:  Max silent contamination: 100.0
29-09-2024 15:05:20:  Searching for marker genes in base database
29-09-2024 15:05:21:  Found 14 marker genes, placing them in the tree using epa-ng
29-09-2024 15:05:50:  Genome belongs to clade: protozoa (Best TaxID: 33846)
29-09-2024 15:05:50:  Searching for marker genes in protozoa database
29-09-2024 15:05:52:  Found 11 marker genes, placing them in the tree using epa-ng
29-09-2024 15:06:01:  Automatically locating best SCMG set
29-09-2024 15:06:28:  ❗The choosen marker gene set is supported by only half (1/11) of the alignments. This generally is an unstable estimate.
29-09-2024 15:06:32:  Searching fasta for selected markers
29-09-2024 15:06:42:  Completeness: 48.67
29-09-2024 15:06:42:  Contamination: 2.65
29-09-2024 15:06:42:  Max silent contamination: 100.0
29-09-2024 15:06:42:  Searching for marker genes in base database
29-09-2024 15:06:42:  No placement marker genes found.
29-09-2024 15:06:42:  Searching for marker genes in base database
29-09-2024 15:06:43:  No placement marker genes found.
29-09-2024 15:06:43:  Searching for marker genes in base database
29-09-2024 15:06:43:  No placement marker genes found.
29-09-2024 15:06:43:  Searching for marker genes in base database
29-09-2024 15:06:43:  No placement marker genes found.
29-09-2024 15:06:43:  Searching for marker genes in base database
29-09-2024 15:06:44:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:06:47:  Genome belongs to clade: protozoa (Best TaxID: 37358)
29-09-2024 15:06:47:  Searching for marker genes in protozoa database
29-09-2024 15:06:48:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:06:50:  Automatically locating best SCMG set
29-09-2024 15:07:17:  Could not identify a single suitable marker gene set
29-09-2024 15:07:17:  No marker gene set could be found with these settings 🙁
Change your parameters and try again?
29-09-2024 15:07:17:  Searching for marker genes in base database
29-09-2024 15:07:17:  No placement marker genes found.
29-09-2024 15:07:17:  Searching for marker genes in base database
29-09-2024 15:07:17:  No placement marker genes found.
29-09-2024 15:07:17:  Searching for marker genes in base database
29-09-2024 15:07:18:  No placement marker genes found.
29-09-2024 15:07:18:  Searching for marker genes in base database
29-09-2024 15:07:18:  No placement marker genes found.
29-09-2024 15:07:18:  Searching for marker genes in base database
29-09-2024 15:07:19:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:07:24:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:07:24:  Searching for marker genes in protozoa database
29-09-2024 15:07:25:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:07:28:  Automatically locating best SCMG set
29-09-2024 15:07:55:  Could not identify a single suitable marker gene set
29-09-2024 15:07:55:  No marker gene set could be found with these settings 🙁
Change your parameters and try again?
29-09-2024 15:07:55:  Searching for marker genes in base database
29-09-2024 15:07:55:  No placement marker genes found.
29-09-2024 15:07:55:  Searching for marker genes in base database
29-09-2024 15:07:55:  No placement marker genes found.
29-09-2024 15:07:55:  Searching for marker genes in base database
29-09-2024 15:07:55:  No placement marker genes found.
29-09-2024 15:07:55:  Searching for marker genes in base database
29-09-2024 15:07:56:  Found 5 marker genes, placing them in the tree using epa-ng
29-09-2024 15:08:04:  Genome belongs to clade: protozoa (Best TaxID: 33634)
29-09-2024 15:08:04:  Searching for marker genes in protozoa database
29-09-2024 15:08:05:  Found 3 marker genes, placing them in the tree using epa-ng
29-09-2024 15:08:08:  Automatically locating best SCMG set
29-09-2024 15:08:46:  ❗The choosen marker gene set is supported by only half (1/3) of the alignments. This generally is an unstable estimate.
29-09-2024 15:08:57:  Searching fasta for selected markers
29-09-2024 15:09:10:  Completeness: 2.0
29-09-2024 15:09:10:  Contamination: 0.4
29-09-2024 15:09:10:  Max silent contamination: 100.0
29-09-2024 15:09:10:  Searching for marker genes in base database
29-09-2024 15:09:10:  No placement marker genes found.
29-09-2024 15:09:10:  Searching for marker genes in base database
29-09-2024 15:09:11:  Found 4 marker genes, placing them in the tree using epa-ng
29-09-2024 15:09:18:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:09:18:  Searching for marker genes in protozoa database
29-09-2024 15:09:18:  Found 5 marker genes, placing them in the tree using epa-ng
29-09-2024 15:09:22:  Automatically locating best SCMG set
29-09-2024 15:09:43:  ❗The choosen marker gene set is supported by only half (2/5) of the alignments. This generally is an unstable estimate.
29-09-2024 15:09:44:  Searching fasta for selected markers
29-09-2024 15:09:45:  Completeness: 25.97
29-09-2024 15:09:45:  Contamination: 1.3
29-09-2024 15:09:45:  Max silent contamination: 100.0
29-09-2024 15:09:45:  Searching for marker genes in base database
29-09-2024 15:09:46:  No placement marker genes found.
29-09-2024 15:09:46:  Searching for marker genes in base database
29-09-2024 15:09:46:  No placement marker genes found.
29-09-2024 15:09:46:  Searching for marker genes in base database
29-09-2024 15:09:47:  Found 7 marker genes, placing them in the tree using epa-ng
29-09-2024 15:10:00:  Genome belongs to clade: protozoa (Best TaxID: 33630)
29-09-2024 15:10:00:  Searching for marker genes in protozoa database
29-09-2024 15:10:01:  Found 11 marker genes, placing them in the tree using epa-ng
29-09-2024 15:10:12:  Automatically locating best SCMG set
29-09-2024 15:10:48:  ❗The choosen marker gene set is supported by only half (4/11) of the alignments. This generally is an unstable estimate.
29-09-2024 15:10:55:  Searching fasta for selected markers
29-09-2024 15:11:11:  Completeness: 48.38
29-09-2024 15:11:11:  Contamination: 4.86
29-09-2024 15:11:11:  Max silent contamination: 100.0
29-09-2024 15:11:11:  Searching for marker genes in base database
29-09-2024 15:11:12:  No placement marker genes found.
29-09-2024 15:11:12:  Searching for marker genes in base database
29-09-2024 15:11:12:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:11:15:  Genome belongs to clade: protozoa (Best TaxID: 31345)
29-09-2024 15:11:15:  Searching for marker genes in protozoa database
29-09-2024 15:11:15:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:11:17:  Automatically locating best SCMG set
29-09-2024 15:11:53:  Searching fasta for selected markers
29-09-2024 15:11:57:  Completeness: 1.4
29-09-2024 15:11:57:  Contamination: 0.0
29-09-2024 15:11:57:  Max silent contamination: 100.0
29-09-2024 15:11:57:  Searching for marker genes in base database
29-09-2024 15:11:58:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:12:01:  Genome belongs to clade: protozoa (Best TaxID: 1242273)
29-09-2024 15:12:01:  Searching for marker genes in protozoa database
29-09-2024 15:12:01:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:12:03:  Automatically locating best SCMG set
29-09-2024 15:12:30:  Could not identify a single suitable marker gene set
29-09-2024 15:12:30:  No marker gene set could be found with these settings 🙁
Change your parameters and try again?
29-09-2024 15:12:30:  Searching for marker genes in base database
29-09-2024 15:12:30:  No placement marker genes found.
29-09-2024 15:12:30:  Searching for marker genes in base database
29-09-2024 15:12:31:  Found 9 marker genes, placing them in the tree using epa-ng
29-09-2024 15:12:42:  Genome belongs to clade: protozoa (Best TaxID: 5878)
29-09-2024 15:12:42:  Searching for marker genes in protozoa database
29-09-2024 15:12:43:  Found 10 marker genes, placing them in the tree using epa-ng
29-09-2024 15:12:47:  Automatically locating best SCMG set
29-09-2024 15:13:16:  ❗The choosen marker gene set is supported by only half (4/10) of the alignments. This generally is an unstable estimate.
29-09-2024 15:13:19:  Searching fasta for selected markers
29-09-2024 15:13:23:  Completeness: 33.02
29-09-2024 15:13:23:  Contamination: 11.16
29-09-2024 15:13:23:  Max silent contamination: 100.0
29-09-2024 15:13:23:  Searching for marker genes in base database
29-09-2024 15:13:24:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:13:28:  Genome belongs to clade: protozoa (Best TaxID: 5738)
29-09-2024 15:13:28:  Searching for marker genes in protozoa database
29-09-2024 15:13:28:  Found 2 marker genes, placing them in the tree using epa-ng
29-09-2024 15:13:31:  Automatically locating best SCMG set
29-09-2024 15:13:47:  Searching fasta for selected markers
29-09-2024 15:13:47:  Completeness: 3.17
29-09-2024 15:13:47:  Contamination: 0.0
29-09-2024 15:13:47:  Max silent contamination: 100.0
29-09-2024 15:13:47:  Searching for marker genes in base database
29-09-2024 15:13:48:  Found 9 marker genes, placing them in the tree using epa-ng
29-09-2024 15:14:07:  Genome belongs to clade: protozoa (Best TaxID: 33846)
29-09-2024 15:14:07:  Searching for marker genes in protozoa database
29-09-2024 15:14:08:  Found 6 marker genes, placing them in the tree using epa-ng
29-09-2024 15:14:14:  Automatically locating best SCMG set
29-09-2024 15:14:41:  Could not identify a single suitable marker gene set
29-09-2024 15:14:41:  No marker gene set could be found with these settings 🙁
Change your parameters and try again?
29-09-2024 15:14:41:  Searching for marker genes in base database
29-09-2024 15:14:41:  No placement marker genes found.
29-09-2024 15:14:41:  Searching for marker genes in base database
29-09-2024 15:14:41:  No placement marker genes found.
29-09-2024 15:14:41:  Searching for marker genes in base database
29-09-2024 15:14:41:  Found 1 marker genes, placing them in the tree using epa-ng
29-09-2024 15:14:45:  Genome belongs to clade: protozoa (Best TaxID: 2699528)
29-09-2024 15:14:45:  Searching for marker genes in protozoa database
29-09-2024 15:14:45:  No placement marker genes found.
29-09-2024 15:14:45:  Searching for marker genes in base database
29-09-2024 15:14:45:  No placement marker genes found.
29-09-2024 15:14:45:  Searching for marker genes in base database
29-09-2024 15:14:46:  Found 3 marker genes, placing them in the tree using epa-ng
29-09-2024 15:14:51:  Genome belongs to clade: protozoa (Best TaxID: protist_common)
29-09-2024 15:14:51:  Searching for marker genes in protozoa database
29-09-2024 15:14:52:  Found 4 marker genes, placing them in the tree using epa-ng
29-09-2024 15:14:55:  Automatically locating best SCMG set
29-09-2024 15:15:22:  ❗The choosen marker gene set is supported by only half (1/4) of the alignments. This generally is an unstable estimate.
29-09-2024 15:15:26:  Searching fasta for selected markers
29-09-2024 15:15:29:  Completeness: 6.64
29-09-2024 15:15:29:  Contamination: 0.44
29-09-2024 15:15:29:  Max silent contamination: 100.0
29-09-2024 15:15:29:  Found 0 large bins to merge with
29-09-2024 15:15:29:  Created 0 merged bins