zoependlington
commented
2 months ago Added as EFO_0022604 and EFO_0022605, which will be available after the EFO May release (15th).
Closed brian-mott closed 2 months ago
zoependlington
commented
2 months ago Added as EFO_0022604 and EFO_0022605, which will be available after the EFO May release (15th).
10x has new versions for the following assays. There should be updated terms for these assays
Preferred term label
10x 3' v4 10x 5' v3
Synonyms
10x 3' v4: 10x 3' v4 sequencing, 10X 3' v4 sequencing, 10X 3' v4 10x 5' v3: 10x 5' v3 sequencing, 10X 5' v3 sequencing, 10X 5' v3
Textual definition
10x 3' v4: 10X 3' v4 is the fourth version of the 10x sequencing technology that sequences from the 3' end of nucleic acid molecules. 10x 5' v3: 10X 5' v3 is the third version of the 10x sequencing technology that sequences from the 5' end of nucleic acid molecules. In the 5' assay, the polyd(T) is supplied in the RT Primer, and the template switch oligo (TSO) is part of the gel bead oligo.
Suggested parent term
10x 3' v4: 10x 3' transcription profiling (EFO:0030003) 10x 5' v3: 10x 5' transcription profiling (EFO:0030004)
https://pages.10xgenomics.com/rs/446-PBO-704/images/10x_LIT000220_Product_Sheet_GEM-X-Single-Cell-Gene-Expression_Letter_Digital.pdf https://pages.10xgenomics.com/rs/446-PBO-704/images/10x_LIT000221_Product_Sheet_GEM-X-Single-Cell-Immune-Profiling_Letter_Digital.pdf