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EDePasquale / DoubletDecon

A tool for removing doublets from single-cell RNA-seq data
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dplyr loading issues #12

Closed chansigit closed 4 years ago

chansigit commented 4 years ago

It took me half an hour to run the Improved_Seurat_Pre_Process function , only to find the function stopped at the last step, telling me I should load the dplyr library.

Could you please load this package in your function when it starts to calculate?

Calculating cluster 0
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Warning message:
"The following arguments are not used: thresh.use"
Error in seuratObject.markers %>% group_by(cluster) %>% top_n(n = num_genes, : could not find function "%>%"
Traceback:

1. Improved_Seurat_Pre_Process(seu_doublet, num_genes = 50, write_files = FALSE)
EDePasquale commented 4 years ago

Hi,

Thank you for bringing this to our attention! We will incorporate this into the beginning of the function in the next update so it should no longer be a problem.

Thanks, Erica