EDePasquale / DoubletDecon

A tool for removing doublets from single-cell RNA-seq data
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issue at step: "combining similar clusters" #2

Closed drhochbaum closed 4 years ago

drhochbaum commented 6 years ago

I think I have successfully pre-processed my data from Seurat using the Seurat_Pre_Process function, but I can't seem to run the Main_Doublet_Decon:

[1] "Reading data..." [1] "Processing raw data..." [1] "Combining similar clusters..." Error in data.frame(..., check.names = FALSE) : arguments imply differing number of rows: 0, 2 In addition: Warning message: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : there is no package called ‘as.color’

Im not sure if the as.color package not being available is causing this issue? (as.color is no longer available for current R version 3.5.1)?

Any help will be much appreciated! Best Daniel

catsargent commented 6 years ago

Hi Daniel,

I also had the same error message. I managed to get around it by downloading the archived version of as.color from here: https://cran.r-project.org/src/contrib/Archive/as.color/

and then installing it from source: install.packages("~/Downloads/as.color_0.1.tar.gz", repos = NULL, type="source")

Regards, Catherine