EDePasquale / DoubletDecon

A tool for removing doublets from single-cell RNA-seq data
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Error in Improved_Seurat_Pre_Process #51

Open mvhunter1 opened 3 years ago

mvhunter1 commented 3 years ago

Hi,

I'm trying to run Improved_Seurat_Pre_Process on a Seurat object that i've used with Seurat v4. I'm getting the following error after the marker-finding step:

NAs introduced by coercionError in if (min(as.numeric(as.character(genes$cluster))) == 0) { : 
  missing value where TRUE/FALSE needed

From reading answers to other issues it seems like the error may be stemming from using idents other than the original Seurat cluster numbers in my Seurat object. Am I still not able to use idents other than the default seurat_clusters? Thank you!