I am trying to run the Main_Doublet_Decon as this:
results <- Main_Doublet_Decon(newFiles$newExpressionFile, newFiles$newGroupsFile, filename, location,
fullDataFile = NULL, removeCC = FALSE, species = "hsa", rhop = 1,
write = TRUE, PMF = TRUE, useFull = FALSE, heatmap = TRUE, centroids=FALSE, num_doubs=200,
only50=FALSE, min_uniq=4, nCores=-1)
But I got errors like this:
Loading packages...
Reading data...
WARNING: if using ICGS2 file input, please import 'rawDataFile' and 'groupsFile' as path/location instead of an R object. Processing raw data... Creating original data heatmap... Combining similar clusters... Creating synthetic doublet profiles...
Step 1: Removing possible doublets...
Step 2: Re-clustering possible doublets...
Step 3: Rescuing cells with unique gene expression...
Error in socketConnection("localhost", port = port, server = TRUE, blocking = TRUE, :
all connections are in use In addition: There were 50 or more warnings (use warnings() to see the first 50)
Hi all-
I am trying to run the Main_Doublet_Decon as this: results <- Main_Doublet_Decon(newFiles$newExpressionFile, newFiles$newGroupsFile, filename, location, fullDataFile = NULL, removeCC = FALSE, species = "hsa", rhop = 1, write = TRUE, PMF = TRUE, useFull = FALSE, heatmap = TRUE, centroids=FALSE, num_doubs=200, only50=FALSE, min_uniq=4, nCores=-1)
But I got errors like this: Loading packages... Reading data... WARNING: if using ICGS2 file input, please import 'rawDataFile' and 'groupsFile' as path/location instead of an R object. Processing raw data... Creating original data heatmap... Combining similar clusters... Creating synthetic doublet profiles... Step 1: Removing possible doublets... Step 2: Re-clustering possible doublets... Step 3: Rescuing cells with unique gene expression... Error in socketConnection("localhost", port = port, server = TRUE, blocking = TRUE, :
all connections are in use In addition: There were 50 or more warnings (use warnings() to see the first 50)
The Seurat version is 4.1.0.
What's wrong with this?