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EMBL-PKU / BASALT

MIT License
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3rd bin selection did not perform, because there is only one assembly! #37

Open Enewmoon opened 4 months ago

Enewmoon commented 4 months ago

Dear @EMBL-PKU ， I have multiple sites, but only one sample per site. So my code is BASALT -a Y_assembly.fa -s Y.1.fastq,Y.fastq -t 64 -m 250

However, when I run to the third step, the following problem appears, can I skip the third step or how to adjust the code?

Basalt_checkpoint.txt 1st autobinner done! 2nd bin selection within group done! 3rd bin selection did not perform, because there is only one assembly!

Looking forward to your early reply.

EMBL-PKU commented 4 months ago

Hi, You do not need to adjust the code for that. The selection module for multiple assemblies could be activated only when there were several assemblies presented. If there is only one assembly, as you already found it, BASALT would skip the step. So do not worry, it is ok

Enewmoon commented 4 months ago

Thank you very much for your answer.

Enewmoon commented 4 months ago

Hello, bother you again, it doesn't seem to run after the third step, but there should be many more steps after that, how to solve this. Looking forward to your early reply. b3b66549a0a77ba935be00a8491dc13

EMBL-PKU commented 4 months ago

Is there only one paired-end reads present in your command? This error may occasionally occurred if only one PE-reads present. I am trying to solve this issue. It may take one day. Please wait until 0607-0608 2024, I will update the new script once I finished.

Enewmoon commented 4 months ago

Is there only one paired-end reads present in your command? This error may occasionally occurred if only one PE-reads present. I am trying to solve this issue. It may take one day. Please wait until 0607-0608 2024, I will update the new script once I finished.

Yes, I only have one paired-end reads present. Thank you again for your reply.