Open thaorope opened 2 years ago
2022-02-07 09:34:37,052 INFO - Running with database db.url = jdbc:hsqldb:mem:f3301075-5e78-4ea0-bd46-51dc6f99f702;shutdown=false;hsqldb.tx=mvcc 2022-02-07 09:34:51,290 INFO - Running migration RenameWorkflowOptionsInMetadata with a read batch size of 100000 and a write batch size of 100000 2022-02-07 09:34:51,339 INFO - [RenameWorkflowOptionsInMetadata] 100% 2022-02-07 09:34:51,597 INFO - Running with database db.url = jdbc:hsqldb:mem:32f55dd3-f0c1-4304-9d26-c8d54a08d98b;shutdown=false;hsqldb.tx=mvcc 2022-02-07 09:34:52,813 INFO - Slf4jLogger started 2022-02-07 09:34:53,219 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - Workflow heartbeat configuration: { "cromwellId" : "cromid-c73d3a1", "heartbeatInterval" : "2 minutes", "ttl" : "10 minutes", "failureShutdownDuration" : "5 minutes", "writeBatchSize" : 10000, "writeThreshold" : 10000 } 2022-02-07 09:34:53,585 WARN - 'docker.hash-lookup.gcr-api-queries-per-100-seconds' is being deprecated, use 'docker.hash-lookup.gcr.throttle' instead (see reference.conf) 2022-02-07 09:34:53,688 cromwell-system-akka.dispatchers.service-dispatcher-16 INFO - WriteMetadataActor configured to flush with batch size 200 and process rate 5 seconds. 2022-02-07 09:34:53,694 cromwell-system-akka.actor.default-dispatcher-8 INFO - KvWriteActor configured to flush with batch size 200 and process rate 5 seconds. 2022-02-07 09:34:53,695 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - CallCacheWriteActor configured to flush with batch size 100 and process rate 3 seconds. 2022-02-07 09:34:53,700 cromwell-system-akka.dispatchers.service-dispatcher-20 INFO - Metadata summary refreshing every 1 second. 2022-02-07 09:34:53,700 cromwell-system-akka.dispatchers.service-dispatcher-20 INFO - No metadata archiver defined in config 2022-02-07 09:34:53,700 cromwell-system-akka.dispatchers.service-dispatcher-20 INFO - No metadata deleter defined in config 2022-02-07 09:34:53,834 cromwell-system-akka.dispatchers.engine-dispatcher-32 INFO - JobExecutionTokenDispenser - Distribution rate: 1 per 2 seconds. 2022-02-07 09:34:54,049 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - SingleWorkflowRunnerActor: Version 65 2022-02-07 09:34:54,083 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - SingleWorkflowRunnerActor: Submitting workflow 2022-02-07 09:34:54,280 cromwell-system-akka.dispatchers.api-dispatcher-34 INFO - Unspecified type (Unspecified version) workflow 69c5d7ab-4e56-4d62-9a66-49f973ae6af3 submitted 2022-02-07 09:34:54,409 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - SingleWorkflowRunnerActor: Workflow submitted UUID(69c5d7ab-4e56-4d62-9a66-49f973ae6af3) 2022-02-07 09:34:54,436 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - 1 new workflows fetched by cromid-c73d3a1: 69c5d7ab-4e56-4d62-9a66-49f973ae6af3 2022-02-07 09:34:54,462 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - WorkflowManagerActor: Starting workflow UUID(69c5d7ab-4e56-4d62-9a66-49f973ae6af3) 2022-02-07 09:34:54,479 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - WorkflowManagerActor: Successfully started WorkflowActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 2022-02-07 09:34:54,479 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - Retrieved 1 workflows from the WorkflowStoreActor 2022-02-07 09:34:54,514 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - WorkflowStoreHeartbeatWriteActor configured to flush with batch size 10000 and process rate 2 minutes. 2022-02-07 09:34:54,805 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - MaterializeWorkflowDescriptorActor [UUID(69c5d7ab)]: Parsing workflow as WDL 1.0 2022-02-07 09:34:58,892 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - Not triggering log of token queue status. Effective log interval = None 2022-02-07 09:35:02,015 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - MaterializeWorkflowDescriptorActor [UUID(69c5d7ab)]: Call-to-Backend assignments: atac.count_signal_track -> slurm, atac.spr -> slurm, atac.jsd -> slurm, atac.pool_ta -> slurm, atac.count_signal_track_pooled -> slurm, atac.pool_blacklist -> slurm, atac.reproducibility_overlap -> slurm, atac.call_peak_ppr2 -> slurm, atac.reproducibility_idr -> slurm, atac.align -> slurm, atac.pool_ta_pr1 -> slurm, atac.bam2ta -> slurm, atac.align_mito -> slurm, atac.call_peak_pr1 -> slurm, atac.preseq -> slurm, atac.idr_pr -> slurm, atac.xcor -> slurm, atac.idr -> slurm, atac.tss_enrich -> slurm, atac.qc_report -> slurm, atac.read_genome_tsv -> slurm, atac.call_peak -> slurm, atac.compare_signal_to_roadmap -> slurm, atac.bam2ta_no_dedup -> slurm, atac.call_peak_pooled -> slurm, atac.overlap_ppr -> slurm, atac.pool_ta_pr2 -> slurm, atac.call_peak_pr2 -> slurm, atac.gc_bias -> slurm, atac.idr_ppr -> slurm, atac.error_input_data -> slurm, atac.fraglen_stat_pe -> slurm, atac.macs2_signal_track -> slurm, atac.frac_mito -> slurm, atac.call_peak_ppr1 -> slurm, atac.annot_enrich -> slurm, atac.filter_no_dedup -> slurm, atac.filter -> slurm, atac.macs2_signal_track_pooled -> slurm, atac.overlap_pr -> slurm, atac.overlap -> slurm 2022-02-07 09:35:02,560 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,562 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,563 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,564 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,570 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,571 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,572 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,572 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,573 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,574 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,575 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,575 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,576 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,577 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,578 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,579 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,582 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,582 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,588 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,589 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,590 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,591 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,593 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,593 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,593 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,594 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,594 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,595 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,596 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,596 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,597 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,612 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,613 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,613 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,613 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,614 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,614 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,615 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,615 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [preemptible, disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,618 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:02,618 cromwell-system-akka.dispatchers.backend-dispatcher-38 WARN - slurm [UUID(69c5d7ab)]: Key/s [disks, docker] is/are not supported by backend. Unsupported attributes will not be part of job executions. 2022-02-07 09:35:07,175 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.read_genome_tsv 2022-02-07 09:35:07,848 cromwell-system-akka.dispatchers.engine-dispatcher-32 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1 2022-02-07 09:35:08,094 cromwell-system-akka.dispatchers.engine-dispatcher-31 INFO - 69c5d7ab-4e56-4d62-9a66-49f973ae6af3-EngineJobExecutionActor-atac.read_genome_tsv:NA:1 [UUID(69c5d7ab)]: Could not copy a suitable cache hit for 69c5d7ab:atac.read_genome_tsv:-1:1. No copy attempts were made. 2022-02-07 09:35:08,170 cromwell-system-akka.dispatchers.backend-dispatcher-41 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: Unrecognized runtime attribute keys: disks, docker 2022-02-07 09:35:08,344 cromwell-system-akka.dispatchers.backend-dispatcher-41 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: `echo "$(basename /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/inputs/-1609375697/hg38_hsv.tsv)" > genome_name
touch ref_fa bowtie2_idx_tar chrsz gensz blacklist blacklist2 touch ref_mito_fa touch bowtie2_mito_idx_tar touch tss tss_enrich # for backward compatibility touch dnase prom enh reg2map reg2map_bed roadmap_meta touch mito_chr_name touch regex_bfilt_peak_chr_name
python <<CODE import os with open('/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/inputs/-1609375697/hg38_hsv.tsv','r') as fp: for line in fp: arr = line.strip('\n').split('\t') if arr: key, val = arr with open(key,'w') as fp2: fp2.write(val) CODE` 2022-02-07 09:35:08,887 cromwell-system-akka.dispatchers.backend-dispatcher-41 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/script fi
EOF
for ITER in 1 2 3
do
sbatch --export=ALL -J cromwell_69c5d7ab_read_genome_tsv -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/stderr \
-p medium --account knipe_dmk2 \
-n 1 --ntasks-per-node=1 --cpus-per-task=1 --mem=2048M --time=780 \
\
/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-read_genome_tsv/execution/script.caper && exit 0
sleep 30
done
exit 1
2022-02-07 09:35:13,720 cromwell-system-akka.dispatchers.backend-dispatcher-41 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: job id: 19415
2022-02-07 09:35:13,726 cromwell-system-akka.dispatchers.backend-dispatcher-43 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: Status change from - to WaitingForReturnCode
2022-02-07 09:35:23,600 cromwell-system-akka.dispatchers.engine-dispatcher-9 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.fraglen_stat_pe
2022-02-07 09:35:23,841 cromwell-system-akka.dispatchers.engine-dispatcher-32 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1
2022-02-07 09:35:23,869 cromwell-system-akka.dispatchers.engine-dispatcher-29 INFO - 69c5d7ab-4e56-4d62-9a66-49f973ae6af3-EngineJobExecutionActor-atac.fraglen_stat_pe:0:1 [UUID(69c5d7ab)]: Could not copy a suitable cache hit for 69c5d7ab:atac.fraglen_stat_pe:0:1. No copy attempts were made.
2022-02-07 09:35:23,869 cromwell-system-akka.dispatchers.backend-dispatcher-46 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: Unrecognized runtime attribute keys: disks, docker
2022-02-07 09:35:23,921 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: set -e python3 $(which encode_task_fraglen_stat_pe.py) \ --nodup-bam /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/inputs/-1090778166/ATRX_KO_4hr_R1_rep2.sub.dedups.sort.bam \ --picard-java-heap 7G
2022-02-07 09:35:23,965 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/script fi
EOF
for ITER in 1 2 3
do
sbatch --export=ALL -J cromwell_69c5d7ab_fraglen_stat_pe -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0 -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/stderr \
-p medium --account knipe_dmk2 \
-n 1 --ntasks-per-node=1 --cpus-per-task=1 --mem=8192M --time=780 \
\
/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-fraglen_stat_pe/shard-0/execution/script.caper && exit 0
sleep 30
done
exit 1
2022-02-07 09:35:28,709 cromwell-system-akka.dispatchers.backend-dispatcher-47 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: job id: 19459
2022-02-07 09:35:28,716 cromwell-system-akka.dispatchers.backend-dispatcher-41 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: Status change from - to WaitingForReturnCode
2022-02-07 09:36:50,791 cromwell-system-akka.dispatchers.backend-dispatcher-41 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.read_genome_tsv:NA:1]: Status change from WaitingForReturnCode to Done
2022-02-07 09:36:57,401 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.bam2ta
2022-02-07 09:36:57,840 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1
2022-02-07 09:36:57,858 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - 69c5d7ab-4e56-4d62-9a66-49f973ae6af3-EngineJobExecutionActor-atac.bam2ta:0:1 [UUID(69c5d7ab)]: Could not copy a suitable cache hit for 69c5d7ab:atac.bam2ta:0:1. No copy attempts were made.
2022-02-07 09:36:57,858 cromwell-system-akka.dispatchers.backend-dispatcher-46 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: Unrecognized runtime attribute keys: disks, docker
2022-02-07 09:36:57,883 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: set -e python3 $(which encode_task_bam2ta.py) \ /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/inputs/-1090778166/ATRX_KO_4hr_R1_rep2.sub.dedups.sort.bam \ --paired-end \ \ --mito-chr-name chrM \ --subsample 0 \ --mem-gb 3.724710758626461 \ --nth 2
2022-02-07 09:36:57,906 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/script fi
EOF
for ITER in 1 2 3
do
sbatch --export=ALL -J cromwell_69c5d7ab_bam2ta -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0 -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/stderr \
-p medium --account knipe_dmk2 \
-n 1 --ntasks-per-node=1 --cpus-per-task=2 --mem=4767M --time=780 \
\
/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-bam2ta/shard-0/execution/script.caper && exit 0
sleep 30
done
exit 1
2022-02-07 09:36:58,710 cromwell-system-akka.dispatchers.backend-dispatcher-71 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: job id: 19645
2022-02-07 09:36:58,714 cromwell-system-akka.dispatchers.backend-dispatcher-69 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: Status change from - to WaitingForReturnCode
2022-02-07 09:36:59,443 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.gc_bias
2022-02-07 09:36:59,839 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1
2022-02-07 09:36:59,848 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - 69c5d7ab-4e56-4d62-9a66-49f973ae6af3-EngineJobExecutionActor-atac.gc_bias:0:1 [UUID(69c5d7ab)]: Could not copy a suitable cache hit for 69c5d7ab:atac.gc_bias:0:1. No copy attempts were made.
2022-02-07 09:36:59,852 cromwell-system-akka.dispatchers.backend-dispatcher-71 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: Unrecognized runtime attribute keys: disks, docker
2022-02-07 09:36:59,881 cromwell-system-akka.dispatchers.backend-dispatcher-71 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: set -e python3 $(which encode_task_gc_bias.py) \ --nodup-bam /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/inputs/-1090778166/ATRX_KO_4hr_R1_rep2.sub.dedups.sort.bam \ --ref-fa /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/inputs/-1609375697/GRCh38_no_alt_analysis_set_GCA_000001405_hsv.15.fasta.gz \ --picard-java-heap 4G
2022-02-07 09:36:59,896 cromwell-system-akka.dispatchers.backend-dispatcher-71 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/script fi
EOF
for ITER in 1 2 3
do
sbatch --export=ALL -J cromwell_69c5d7ab_gc_bias -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0 -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/stderr \
-p medium --account knipe_dmk2 \
-n 1 --ntasks-per-node=1 --cpus-per-task=1 --mem=4767M --time=780 \
\
/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/execution/script.caper && exit 0
sleep 30
done
exit 1
2022-02-07 09:37:03,710 cromwell-system-akka.dispatchers.backend-dispatcher-71 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: job id: 19664
2022-02-07 09:37:03,715 cromwell-system-akka.dispatchers.backend-dispatcher-69 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: Status change from - to WaitingForReturnCode
2022-02-07 09:37:04,524 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.jsd
2022-02-07 09:37:05,841 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1
2022-02-07 09:37:05,852 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - 69c5d7ab-4e56-4d62-9a66-49f973ae6af3-EngineJobExecutionActor-atac.jsd:NA:1 [UUID(69c5d7ab)]: Could not copy a suitable cache hit for 69c5d7ab:atac.jsd:-1:1. No copy attempts were made.
2022-02-07 09:37:05,852 cromwell-system-akka.dispatchers.backend-dispatcher-46 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: Unrecognized runtime attribute keys: disks, docker
2022-02-07 09:37:05,873 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: set -e python3 $(which encode_task_jsd.py) \ /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/inputs/-1090778166/ATRX_KO_4hr_R1_rep2.sub.dedups.sort.bam \ --mapq-thresh 30 \ --blacklist /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/inputs/-1609375697/hg38.blacklist.bed.gz \ --nth 4
2022-02-07 09:37:05,887 cromwell-system-akka.dispatchers.backend-dispatcher-46 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/script fi
EOF
for ITER in 1 2 3
do
sbatch --export=ALL -J cromwell_69c5d7ab_jsd -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/stderr \
-p medium --account knipe_dmk2 \
-n 1 --ntasks-per-node=1 --cpus-per-task=4 --mem=5343M --time=780 \
\
/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-jsd/execution/script.caper && exit 0
sleep 30
done
exit 1
2022-02-07 09:37:08,718 cromwell-system-akka.dispatchers.backend-dispatcher-71 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: job id: 19699
2022-02-07 09:37:08,725 cromwell-system-akka.dispatchers.backend-dispatcher-70 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: Status change from - to WaitingForReturnCode
2022-02-07 09:47:02,971 cromwell-system-akka.dispatchers.backend-dispatcher-176 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.fraglen_stat_pe:0:1]: Status change from WaitingForReturnCode to Done
2022-02-07 09:51:28,087 cromwell-system-akka.dispatchers.backend-dispatcher-226 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.jsd:NA:1]: Status change from WaitingForReturnCode to Done
2022-02-07 09:52:19,290 cromwell-system-akka.dispatchers.backend-dispatcher-261 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:1]: Status change from WaitingForReturnCode to Done
2022-02-07 09:52:23,719 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Retrying job execution for atac.gc_bias:0:2
2022-02-07 09:52:23,981 cromwell-system-akka.dispatchers.engine-dispatcher-10 INFO - WorkflowExecutionActor-69c5d7ab-4e56-4d62-9a66-49f973ae6af3 [UUID(69c5d7ab)]: Starting atac.gc_bias
2022-02-07 09:52:25,831 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - Assigned new job execution tokens to the following groups: 69c5d7ab: 1
2022-02-07 09:52:25,857 cromwell-system-akka.dispatchers.backend-dispatcher-261 WARN - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: Unrecognized runtime attribute keys: disks, docker
2022-02-07 09:52:25,883 cromwell-system-akka.dispatchers.backend-dispatcher-261 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: set -e python3 $(which encode_task_gc_bias.py) \ --nodup-bam /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/inputs/-1090778166/ATRX_KO_4hr_R1_rep2.sub.dedups.sort.bam \ --ref-fa /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/inputs/-1609375697/GRCh38_no_alt_analysis_set_GCA_000001405_hsv.15.fasta.gz \ --picard-java-heap 4G
2022-02-07 09:52:25,919 cromwell-system-akka.dispatchers.backend-dispatcher-261 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: executing: cat << EOF > /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/script.caper
if [ 'true' == 'true' ] && [ 'conda' == 'singularity' ] || \
[ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' ]
then
mkdir -p $HOME/.singularity/lock/
flock --exclusive --timeout 600 \
$HOME/.singularity/lock/echo -n 'https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif' | md5sum | cut -d' ' -f1
\
singularity exec --containall https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif echo 'Successfully pulled https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif'
singularity exec --cleanenv --home=`dirname /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2` \
--bind=, \
\
https://encode-pipeline-singularity-image.s3.us-west-2.amazonaws.com/atac-seq-pipeline_v2.0.3.sif /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/script
elif [ 'true' == 'true' ] && [ 'conda' == 'conda' ] || \ [ 'true' == 'false' ] && [ 'true' == 'true' ] && [ ! -z 'encode-atac-seq-pipeline' ] then conda run --name=encode-atac-seq-pipeline /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/script
else /bin/bash /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/script fi
EOF
for ITER in 1 2 3 do sbatch --export=ALL -J cromwell_69c5d7ab_gc_bias -D /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2 -o /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/stdout -e /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/stderr \ -p medium --account knipe_dmk2 \ -n 1 --ntasks-per-node=1 --cpus-per-task=1 --mem=4767M --time=780 \ \ /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/script.caper && exit 0 sleep 30 done exit 1 2022-02-07 09:52:28,701 cromwell-system-akka.dispatchers.backend-dispatcher-275 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: job id: 29676 2022-02-07 09:52:28,704 cromwell-system-akka.dispatchers.backend-dispatcher-278 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: Status change from - to WaitingForReturnCode 2022-02-07 10:10:22,926 cromwell-system-akka.dispatchers.backend-dispatcher-480 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.gc_bias:0:2]: Status change from WaitingForReturnCode to Done 2022-02-07 10:34:20,682 cromwell-system-akka.dispatchers.backend-dispatcher-513 INFO - BackgroundConfigAsyncJobExecutionActor [UUID(69c5d7ab)atac.bam2ta:0:1]: Status change from WaitingForReturnCode to Done 2022-02-07 10:34:21,347 cromwell-system-akka.dispatchers.engine-dispatcher-29 INFO - WorkflowManagerActor: Workflow 69c5d7ab-4e56-4d62-9a66-49f973ae6af3 failed (during ExecutingWorkflowState): Job atac.gc_bias:0:2 exited with return code 1 which has not been declared as a valid return code. See 'continueOnReturnCode' runtime attribute for more details. Check the content of stderr for potential additional information: /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/execution/stderr. [First 3000 bytes]:Picked up _JAVA_OPTIONS: -Djava.io.tmpdir=/n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/tmp.d45903f0 INFO 2022-02-07 10:03:19 CollectGcBiasMetrics
** NOTE: Picard's command line syntax is changing.
** For more information, please see: ** https://github.com/broadinstitute/picard/wiki/Command-Line-Syntax-Transition-For-Users-(Pre-Transition)
** The command line looks like this in the new syntax:
** CollectGcBiasMetrics -R /n/scratch3/users/t/tht900/atac-testing/atac/69c5d7ab-4e56-4d62-9a66-49f973ae6af3/call-gc_bias/shard-0/attempt-2/inputs/-1609375697/GRCh38_no_alt_analysis_set_GCA_000001405_hsv.15.fasta.gz -I ./ATRX_KO_4hr_R1_rep2.sub.dedups.sort.no_rg.bam -O ATRX_KO_4hr_R1_rep2.sub.dedups.sort.gc.txt -USE_JDK_DEFLATER TRUE -USE_JDK_INFLATER TRUE -VERBOSITY ERROR -QUIET TRUE -ASSUME_SORTED FALSE -CHART ATRX_KO_4hr_R1_rep2.sub.dedups.sort.gcPlot.pdf -S ATRX_KO_4hr_R1_rep2.sub.dedups.sort.gcSummary.txt
Exception in thread "main" java.lang.ArrayIndexOutOfBoundsException: Index 17009 out of bounds for length 16570
at picard.analysis.GcBiasMetricsCollector.addRead(GcBiasMetricsCollector.java:384)
at picard.analysis.GcBiasMetricsCollector.access$600(GcBiasMetricsCollector.java:48)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.addReadToGcData(GcBiasMetricsCollector.java:221)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.acceptRecord(GcBiasMetricsCollector.java:155)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.acceptRecord(GcBiasMetricsCollector.java:100)
at picard.metrics.MultiLevelCollector$AllReadsDistributor.acceptRecord(MultiLevelCollector.java:192)
at picard.metrics.MultiLevelCollector.acceptRecord(MultiLevelCollector.java:315)
at picard.analysis.CollectGcBiasMetrics.acceptRead(CollectGcBiasMetrics.java:172)
at picard.analysis.SinglePassSamProgram.makeItSo(SinglePassSamProgram.java:145)
at picard.analysis.SinglePassSamProgram.doWork(SinglePassSamProgram.java:84)
at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:305)
at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:103)
at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:113)
Traceback (most recent call last):
File "/home/tht900/miniconda3/envs/encode-atac-seq-pipeline/bin/encode_task_gc_bias.py", line 143, in
2022-02-07 10:34:23,717 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - WorkflowManagerActor: Workflow actor for 69c5d7ab-4e56-4d62-9a66-49f973ae6af3 completed with status 'Failed'. The workflow will be removed from the workflow store. 2022-02-07 10:35:15,061 cromwell-system-akka.dispatchers.engine-dispatcher-11 INFO - SingleWorkflowRunnerActor workflow finished with status 'Failed'. 2022-02-07 10:35:19,080 cromwell-system-akka.dispatchers.engine-dispatcher-33 INFO - SingleWorkflowRunnerActor writing metadata to /n/scratch3/users/t/tht900/atac-testing/caper_temp/atac_medium_2/20220207_093423_447456/metadata.json 2022-02-07 10:35:19,147 INFO - Workflow polling stopped 2022-02-07 10:35:19,152 INFO - 0 workflows released by cromid-c73d3a1 2022-02-07 10:35:19,156 INFO - Shutting down WorkflowStoreActor - Timeout = 5 seconds 2022-02-07 10:35:19,166 INFO - Shutting down WorkflowLogCopyRouter - Timeout = 5 seconds 2022-02-07 10:35:19,169 cromwell-system-akka.dispatchers.engine-dispatcher-30 INFO - Aborting all running workflows. 2022-02-07 10:35:19,173 INFO - WorkflowStoreActor stopped 2022-02-07 10:35:19,191 INFO - Shutting down JobExecutionTokenDispenser - Timeout = 5 seconds 2022-02-07 10:35:19,193 INFO - JobExecutionTokenDispenser stopped 2022-02-07 10:35:19,196 INFO - WorkflowLogCopyRouter stopped 2022-02-07 10:35:19,197 INFO - Shutting down WorkflowManagerActor - Timeout = 3600 seconds 2022-02-07 10:35:19,198 cromwell-system-akka.dispatchers.engine-dispatcher-30 INFO - WorkflowManagerActor: All workflows finished 2022-02-07 10:35:19,201 INFO - WorkflowManagerActor stopped 2022-02-07 10:35:19,744 INFO - Connection pools shut down 2022-02-07 10:35:19,744 INFO - Shutting down SubWorkflowStoreActor - Timeout = 1800 seconds 2022-02-07 10:35:19,745 INFO - Shutting down JobStoreActor - Timeout = 1800 seconds 2022-02-07 10:35:19,746 INFO - Shutting down CallCacheWriteActor - Timeout = 1800 seconds 2022-02-07 10:35:19,748 INFO - CallCacheWriteActor Shutting down: 0 queued messages to process 2022-02-07 10:35:19,750 INFO - Shutting down ServiceRegistryActor - Timeout = 1800 seconds 2022-02-07 10:35:19,751 INFO - Shutting down DockerHashActor - Timeout = 1800 seconds 2022-02-07 10:35:19,808 INFO - Shutting down connection pool: curAllocated=0 idleQueues.size=0 waitQueue.size=0 maxWaitQueueLimit=256 closed=false 2022-02-07 10:35:19,810 INFO - Shutting down connection pool: curAllocated=0 idleQueues.size=0 waitQueue.size=0 maxWaitQueueLimit=256 closed=false 2022-02-07 10:35:19,811 INFO - Shutting down connection pool: curAllocated=0 idleQueues.size=0 waitQueue.size=0 maxWaitQueueLimit=256 closed=false 2022-02-07 10:35:19,818 INFO - Shutting down connection pool: curAllocated=0 idleQueues.size=0 waitQueue.size=0 maxWaitQueueLimit=256 closed=false 2022-02-07 10:35:19,820 INFO - Shutting down IoProxy - Timeout = 1800 seconds 2022-02-07 10:35:19,827 INFO - SubWorkflowStoreActor stopped 2022-02-07 10:35:19,829 INFO - JobStoreActor stopped 2022-02-07 10:35:19,830 INFO - CallCacheWriteActor stopped 2022-02-07 10:35:19,830 INFO - DockerHashActor stopped 2022-02-07 10:35:19,830 INFO - IoProxy stopped 2022-02-07 10:35:19,832 INFO - WriteMetadataActor Shutting down: 0 queued messages to process 2022-02-07 10:35:19,833 INFO - KvWriteActor Shutting down: 0 queued messages to process 2022-02-07 10:35:19,843 INFO - ServiceRegistryActor stopped 2022-02-07 10:35:19,868 INFO - Database closed 2022-02-07 10:35:19,868 INFO - Stream materializer shut down 2022-02-07 10:35:19,870 INFO - WDL HTTP import resolver closed
If you don't start from FASTQs make sure that genome reference data defined in /home/tht900/ATAC-seq-testing/hg38_hsv_genome_database3/hg38_hsv.tsv
match with those used for aligning the BAM file ATRX_KO_4hr_R1_rep2.sub.dedups.bam
.
Did you test the pipeline with our test example?
@leepc12 : Thank you for replying!
I tested the pipeline with your test example, and it worked well.
For my sample:
I don't quite understand what you mean by "geome reference data matching those used for alignment the BAM file". Could you please elaborate on this?
Thank you for your help!
Hi @leepc12 : I re-ran this again multiple times, and still got the same errors. I can't seem to figure out what is going wrong here. The pipeline ran well with the test example. Do you have any idea where I should look into with my dataset?
Please try with pipeline's own subsample feature.
Start from FASTQs and add something like "atac.subsample_reads": 1000000
to your input JSON.
Describe the bug
Hi team,
I tried to peak-call using input files as dedupped BAM files. It worked initially for 1 sample (2 replicates), but not for the second sample.
OS/Platform
Caper configuration file
Paste contents of
~/.caper/default.conf
.Input JSON file
Paste contents of your input JSON file.
Troubleshooting result
If you ran
caper run
without Caper server then Caper automatically runs a troubleshooter for failed workflows. Find troubleshooting result in the bottom of Caper's screen log.If you ran
caper submit
with a running Caper server then first find your workflow ID (1st column) withcaper list
and runcaper debug [WORKFLOW_ID]
.Paste troubleshooting result.