Taylor primers are from Taylor et al. 2016. They are called 5.8S and ITS4-FUN
Main reasons we use these primers are:
Fungi-specific with little-to-no binding to plants, protists, or other eukaryotes (this is an issue with the popular ITS1/ITS2 primer set). In particular, ITS2 hits plants common to arctic habitats such as lingon berries, betula - though this is likely because the soils that were tested had these plants, so it's possible and even likely that other plants may be hit by the ITS2 primer.
Reduces the amplicon variable length issue by shifting the forward primer downstream of the intron-insertion site just downstream of the classic ITS1 primer
Was tested on both mock communities, but also on real world soil communities from boreal forests.
Primer sequences are:
AACTTTYRRCAAYGGATCWCT for 5.8S-Fun
AGCCTCCGCTTATTGATATGCTTAART for ITS4-Fun
To be used in the tutorial properly, they should be reverse complemented as this is how they are arranged on our barcodes.
Taylor primers are from Taylor et al. 2016. They are called 5.8S and ITS4-FUN
Main reasons we use these primers are:
Primer sequences are:
AACTTTYRRCAAYGGATCWCTfor 5.8S-FunAGCCTCCGCTTATTGATATGCTTAARTfor ITS4-FunTo be used in the tutorial properly, they should be reverse complemented as this is how they are arranged on our barcodes.