FPetitprez / webMCP-counter

Shiny app to run MCP-counter and mMCP-counter
GNU General Public License v3.0
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Disconnected from the server. Reload #8

Closed CRubioCiemat closed 2 years ago

CRubioCiemat commented 3 years ago

Describe the bug A clear and concise description of what the bug is.

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  4. See error

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FPetitprez commented 3 years ago

Hello, and thank you for using webMCP-counter. To help me identify the possible error, could you indicate me the following details: 1) OS and web browser you are working on (Windows/Mac/Linux and Firefox/Chrome/...)

2) How is your gene expression data formatted (text file, excel speadsheet)

3) What is the format of the gene IDs (Gene symbol, ENSEMBL)? If you don't know, give me examples of genes from your dataset I can deduce it from this.

4) Are you trying to run it on mouse or human data?

5) Are the samples in columns and the genes in rows? Is the first column of the file the gene IDs?

6) Can you tell me the steps you followed that lead to this error?

CRubioCiemat commented 3 years ago

Sorry, I HAVE NOT GIVEN DETAILS.

Hello, and thank you for using webMCP-counter. To help me identify the possible error, could you indicate me the following details:

  1. OS and web browser you are working on (Windows/Mac/Linux and Firefox/Chrome/...)--> Windows
  2. How is your gene expression data formatted (text file, excel speadsheet)--> text file
  3. What is the format of the gene IDs (Gene symbol, ENSEMBL)? If you don't know, give me examples of genes from your dataset I can deduce it from this. -->Gene Symbol
  4. Are you trying to run it on mouse or human data? --> in mouse
  5. Are the samples in columns and the genes in rows?-->The samples are in columns and genes in rows Is the first column of the file the gene IDs?--> The first column are Gene Symbol
  6. Can you tell me the steps you followed that lead to this error? I upload the gene expression profiles file, I selected the txt format, gene symbol and mouse, and I clic Run
FPetitprez commented 3 years ago

What is the separator used in your file? Do you mind emailing me an example of your data ?

FPetitprez commented 3 years ago

Hi again. In the sample you gave me, there are 2 things that cause errors:

  1. There were many empty lines at the end of your file. There must be no empty line.
  2. There was no gene from the mMCP-counter signatures. You need at least one gene of a signature to be present in your data, otherwise it cannot be estimated. If no signature at all can be estimated, this gives an error. The signatures can be found in table S1 of the mMCP-counter article: https://link.springer.com/article/10.1186/s13073-020-00783-w#additional-information
FPetitprez commented 3 years ago

What technology was your data generated with? RNA-seq?

CRubioCiemat commented 3 years ago

Hello,

Sorry for not answering before. I can check empty lines. My data was generated by Transcriptome Array 1.0 Clarion Mouse. I can't review it right now, but as soon as I do I try again.

Thank’s! c

De: FPetitprez @. Enviado el: viernes, 23 de abril de 2021 16:36 Para: FPetitprez/webMCP-counter @.> CC: Rubio Martin, Carolina @.>; Author @.> Asunto: Re: [FPetitprez/webMCP-counter] Disconnected from the server. Reload (#8)

What technology was your data generated with? RNA-seq?

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