Closed zhangqc723 closed 7 months ago
My guess is that this has to do with the gzip
compression, together with the--cores 8
. If trimmed sequences get reported in a different order due to multi-core processing, the compressed files do not necessarily have the same file size.
I run TrimGalore twice as follow code, trim_galore \ --cores 8 --quality 20 --stringency 3 \ --paired --phred33 \ --output_dir /test \ R1.fq.gz R2.fq.gz And I view the md5 value of output from same R1 and R2 file, but the value is different. Could you tell me why? Thanks!