Open dimalvovs opened 2 months ago
Xenium comments addressed in d17a538. The conda environment usage is removed because it was a holdover from an initial attempt to use Leiden clustering. The presented scripts use Louvain clustering.
Commented out code removed in 091299b
Here is the reviewer's comment:
The provided source code does not include any documentation regarding how should the code by used, in what order, under which software requirements and includes hardcoded paths to the authors' machine, making it unusable and hard to scrutinize. Improving the code would enable reproduction of the results and broad use of the analytical approach since the paper is mainly about bringing forth a new apporoach. Furthermore, I would recommend also depositing the source code in a openly accessibly, independent repository for archival such as Zenodo, which can be done easily by connecting the Github repositorie(s) to Zenodo.
Code documentation improvements
visium analysis scripts
[x] possibly non-existant variable Grade_pal
[x] are these comments needed? https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/00_PanIN_Custom_Functions.R#L1031 https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/00_PanIN_Custom_Functions.R#L483 https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/00_PanIN_Custom_Functions.R#L724 https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/00_PanIN_Custom_Functions.R#L755
[x] fix broken source paths, for example https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/01_Pre_processing_paired_cohort.R#L26 https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/02_Paired_cohort_visium_analysis.R#L26
[x] folder structure and path updates https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/01_Pre_processing_paired_cohort.R#L144 https://github.com/FertigLab/PanIN_carcinogeneisis_spatial_analysis/blob/52f6c9c010ff116490510f4d1a834567cbc0198e/scripts/visium_analysis/PDAC_atlas_projection/01_Pre_processing_paired_cohort.R#L163
xenium analysis scripts
closing