new split protein splicing tag terms - for the CfaC and CfaN split inteins used in the siTrojan-GAL4 constructs in FBrf0259289 (PMID:38602904)
This request uses the new split gene product engineering tool term requested in #240
1. new term:
[Term]
id: FBcv:NEW
name: split protein splicing tag
namespace: experimental_tool_descriptor
def: "Sequence that forms part of the protein product encoded by a transgenic locus or modified endogenous locus and that can catalyze protein splicing when brought together with a complementary split protein splicing tag in vivo. Typically used to join two separate protein sequences by trans-splicing; in this case the two separate protein sequences are each initially tagged with one half of a pair of complementary split protein splicing tags. Alternatively, if both halves of a pair of complementary split protein splicing tags are used to tag each end of a single protein sequence, they can be used to generate a cyclized protein molecule." [FBC:GM]
is_a: FBcv:NEW ! split gene product engineering tool
2. tweak to definition of parent split system component term so that new split protein tag terms fit properly under it.
id: FBcv:0005051
name: split system component
change:
def: "Component that forms part of a 'split system'. A split system component corresponds to a non-functional fragment of a functional experimental tool that would normally be encoded by a single gene product. When combined with another appropriate split system component in vivo, a functional experimental tool is produced. The fragments of a split system can be brought together in vivo in a number of different ways; for example, by fusing fragments to peptides that physically interact in vivo." [FBC:GM]
to:
def: "Component that forms part of a 'split system'. A split system component corresponds to a non-functional fragment of a functional experimental tool. When combined with another appropriate split system component in vivo, a functional experimental tool is produced. The fragments of a split system can be brought together in vivo in a number of different ways; for example, by fusing fragments to peptides that physically interact in vivo." [FBC:GM]
new
split protein splicing tagterms - for the CfaC and CfaN split inteins used in the siTrojan-GAL4 constructs in FBrf0259289 (PMID:38602904)This request uses the new
split gene product engineering toolterm requested in #2401. new term:
[Term] id: FBcv:NEW name: split protein splicing tag namespace: experimental_tool_descriptor def: "Sequence that forms part of the protein product encoded by a transgenic locus or modified endogenous locus and that can catalyze protein splicing when brought together with a complementary split protein splicing tag in vivo. Typically used to join two separate protein sequences by trans-splicing; in this case the two separate protein sequences are each initially tagged with one half of a pair of complementary split protein splicing tags. Alternatively, if both halves of a pair of complementary split protein splicing tags are used to tag each end of a single protein sequence, they can be used to generate a cyclized protein molecule." [FBC:GM] is_a: FBcv:NEW ! split gene product engineering tool
2. tweak to definition of parent split system component term so that new split protein tag terms fit properly under it.
id: FBcv:0005051 name: split system component
change:
def: "Component that forms part of a 'split system'. A split system component corresponds to a non-functional fragment of a functional experimental tool that would normally be encoded by a single gene product. When combined with another appropriate split system component in vivo, a functional experimental tool is produced. The fragments of a split system can be brought together in vivo in a number of different ways; for example, by fusing fragments to peptides that physically interact in vivo." [FBC:GM]
to:
def: "Component that forms part of a 'split system'. A split system component corresponds to a non-functional fragment of a functional experimental tool. When combined with another appropriate split system component in vivo, a functional experimental tool is produced. The fragments of a split system can be brought together in vivo in a number of different ways; for example, by fusing fragments to peptides that physically interact in vivo." [FBC:GM]