Closed notmaurox closed 2 years ago
There is utility to a pvalue calculation that only uses negative control intensity when constructing the ECDF. For example - when a chip section is empty (due to operator or automation error), 0% of probes for a sample will pass this pvalue check.
SeSAMe currently supports this functionality: https://github.com/zwdzwd/sesame/blob/master/R/detection.R#L73
There is utility to a pvalue calculation that only uses negative control intensity when constructing the ECDF. For example - when a chip section is empty (due to operator or automation error), 0% of probes for a sample will pass this pvalue check.
SeSAMe currently supports this functionality: https://github.com/zwdzwd/sesame/blob/master/R/detection.R#L73