mohakpatel
commented
8 years ago The algorithm doesn't inherently have a bias for error in a particular direction. It is symmetric for all the directions (x,y and z). However, the issues come from the images being used. In confocal images, there is a larger spread in PSF in z-direction compared to x and y directions. Higher errors in z-direction will arise from the images, and not the algorithm itself. Deconvolving images to correct for PSF before using FIDVC will help you reduce errors in z-directions. How are you taking images? This might help figure out the source of error.
Hello again,
Have you performed any studies comparing the accuracy of the algorithm in the xy vs. z directions? Ideally, accuracy would be equivalent in all directions, but results of mine indicate that xy error is approximately 10-20% while z error is 30-40%. I suspect that this is likely due to the nature of reconstructing 3D volumes from slices in the z direction that results in lower accuracy in this direction. Have you made similar observations or is it possible that something may be wrong with my system? Thanks!