Closed oyxf closed 1 year ago
Hi oyxf,
For OSCC_12 invadeseq data (single-cell data with enriched bacterial sequence), could you please try:
curl -L ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR181/079/SRR18185479/SRR18185479.fastq.gz -o OSCC_12_S5_L001_R2_001.fastq.gz
curl -L ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR181/080/SRR18185480/SRR18185480.fastq.gz -o OSCC_12_S5_L001_R1_001.fastq.gz
curl -L ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR181/081/SRR18185481/SRR18185481.fastq.gz -o OSCC_12_S5_L001_I1_001.fastq.gz
Hanrui
Hi oyxf,
I'm going to close this issue, please feel free to reopen it if you have any other questions!
Hanrui
Sorry for the late reply。The new data works good. Thank you very much !!
Dear Hanrui, @hanruiw I encountered the same issue as oyxf, I tried running cellranger on the files downloaded in the way you suggested. I get the following error:
[error] Pipestance failed. Error log at:
OSCC_12/SC_RNA_COUNTER_CS/SC_MULTI_CORE/MULTI_CHEMISTRY_DETECTOR/DETECT_COUNT_CHEMISTRY/fork0/chnk0-uebcbc38319/_errors
Log message:
FASTQ header mismatch detected at line 4 of input files "/data/PRJNA811533_INVADEseq/OSCC12/OSCC_12_S5_L001_R1_001.fastq.gz" and "/data/PRJNA811533_INVADEseq/OSCC12/OSCC_12_S5_L001_R2_001.fastq.gz": file: "/data/PRJNA811533_INVADEseq/OSCC12/OSCC_12_S5_L001_R1_001.fastq.gz", line: 4
I run the following cellranger command:
cellranger count --id=OSCC_12 \
--transcriptome=/cellranger/refdata-gex-GRCh38-2020-A \
--fastqs=/data/PRJNA811533_INVADEseq/OSCC12 \
--sample=OSCC_12\
--include-introns true \
--localcores=8 \
--localmem=62\
--chemistry=SC5P-PE
Thanks in advance, Elinor
Dear Elinor,
Thank you for your interest in our paper. I think this might because OSCC_12 invadeseq data is associated with three SRR IDs (SRR18185479, SRR18185480, SRR18185481), thus read names in read files are not matched toward each other. Could you please try to match the read names and rerun CellRanger?
Best, Hanrui
Dear Hanrui, @hanruiw
Thank you very much for your reply and help. When I setted -chemistry=SC5P-PE as your suggestion, an error appeared as follow :
I used this command to convert OSCC_12 sra to fastq :
OSCC_12_fastqs:
And run cellranger cmd as follow :
Thanks in advance, oyxf