FrederickHuangLin / ANCOMBC

Differential abundance (DA) and correlation analyses for microbial absolute abundance data
https://www.nature.com/articles/s41467-020-17041-7
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Pseudo-count sensitivity testing when no pseudo-counts are added #256

Open vmaffei opened 7 months ago

vmaffei commented 7 months ago

Dear FrederickHuangLin,

Thank you for developing this software and making it available on github! If you have a moment, would you mind commenting on the benefits of sensitivity testing for pseudo-count addition when pseudo-counts are not being added to the data? I see in the manuscript that the FDR is more robustly controlled with this testing, so that is a plus. However, if pseudo-counts are not being added to the data, are the results of this sensitivity test still relevant? I apologize if these questions have been asked elsewhere and greatly appreciate your time with this.

Vince