Closed skoepper closed 11 months ago
I believe this question has been addressed by email. For other readers who have a similar question, I copied my reply in below:
To address your specific questions:
The first point seems to pertain more to the capabilities of ANCOM rather than its underlying assumptions. If my recollection serves me correctly, ANCOM 2.1 does indeed support nonparametric tests.
The ANCOM model relies on two fundamental assumptions you found to ensure the validity of statistical inferences. These assumptions are integral to the inherent formula of ANCOM. When discussing assumptions, it's crucial to connect them with the methodology rather than the code implementation. The code itself serves as a means of executing the methodology and thus must adhere to the underlying assumptions of ANCOM for making conclusions.
Best regards, Huang
Hello,
I am using ancom 2.1 for the differential abundance analysis of my microbiome data. To understand how the method work, I read the supplementary material of Mandal et al. (2015), and it states that there are three assumptions for ancom:
Based on the code I believe assumption 2b is used, but I would be happy for a clarification on this.
Thanks, Svenja