CLE Lab: Add index selection in all library construction protocols

[tim-snyder-wustl]

Waiting on more info from Andy.

• Need index integration in all library construction protocols - still thinking that index name might be best for picking but if we could add well location to name that would be nice
• Need to update Index DB with CLE barcodes (new column/property).

[abohanno]

I will try and work on adding index barcodes. If we could pick by barcode but then have it show us barcode, index name, and sequence that would be ideal

[LMT337]

Reached out to Andy to add barcod columns to index sheet: https://wustl.app.box.com/file/1009899994712 Asked for clarification on columns to pull in to ESP.

[LMT337]

Library Construction Workflows: CLE Lab: EpMotion Dilutions for Library Construction No Construction Protocol

CLE Lab: Haloplex Library Construction Protocol: CLE Lab: Haloplex Library Construction

CLE Lab: Targeted Capture Library Construction Protocol: CLE Lab: KAPA Hyper Library Construction

CLE Lab: Whole Genome Library Construction Protocol: CLE Lab: ILMN Library Construction

[abohanno]

CLE_IndexSequences.xlsx

[LMT337]

Need index ingest process, plate barcode, 2d barcodes to well position for master tray. Tray gets aliquoted into temp index tray. For index selection, there are three trays, DNA, index and destination. Plate scan those into a workflow sheet. Also way to manually seclect index if plate scanner is not available.

[LMT337]

WGS uses master tray, no aliquot tray.

[tim-snyder-wustl]

For Webtools:

• add option to 'decommision' plates - mark as 'empty' in the UI
• 2D barcodes will change, but indexes stay the same in each well
• exome master tray is static, gets used only once, the aliquot tray gets used ~18 times
• need to add 2D barcodes to each well in firestore