CLE Lab: Add index selection in all library construction protocols

GTAC-MGI / GTAC-ESP-LIMS

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CLE Lab: Add index selection in all library construction protocols #210

Open tim-snyder-wustl opened 1 year ago

tim-snyder-wustl commented 1 year ago

Waiting on more info from Andy.

Need index integration in all library construction protocols - still thinking that index name might be best for picking but if we could add well location to name that would be nice
Need to update Index DB with CLE barcodes (new column/property).

abohanno commented 1 year ago

I will try and work on adding index barcodes. If we could pick by barcode but then have it show us barcode, index name, and sequence that would be ideal

LMT337 commented 1 year ago

Reached out to Andy to add barcod columns to index sheet: https://wustl.app.box.com/file/1009899994712 Asked for clarification on columns to pull in to ESP.

LMT337 commented 1 year ago

Library Construction Workflows: CLE Lab: EpMotion Dilutions for Library Construction No Construction Protocol

CLE Lab: Haloplex Library Construction Protocol: CLE Lab: Haloplex Library Construction

CLE Lab: Targeted Capture Library Construction Protocol: CLE Lab: KAPA Hyper Library Construction

CLE Lab: Whole Genome Library Construction Protocol: CLE Lab: ILMN Library Construction

abohanno commented 4 months ago

CLE_IndexSequences.xlsx

LMT337 commented 2 weeks ago

Need index ingest process, plate barcode, 2d barcodes to well position for master tray. Tray gets aliquoted into temp index tray. For index selection, there are three trays, DNA, index and destination. Plate scan those into a workflow sheet. Also way to manually seclect index if plate scanner is not available.

LMT337 commented 2 weeks ago

WGS uses master tray, no aliquot tray.