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GTAC-MGI / GTAC-ESP-LIMS

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Dilution #23

Closed tim-snyder-wustl closed 2 years ago

tim-snyder-wustl commented 2 years ago

Clone Manual Dilution steps from RNA workflow in Seq Pooling (SC does not do any library pooling, however).

LMT337 commented 2 years ago

Example_10nM dilution.xls

tim-snyder-wustl commented 2 years ago

Dilution Calculations:

sample name
DNA ng/μL: number
size in bp: number
DNA nM: (DNA ng/μL * (10^3/1) * (1/649) * (1/size in bp)) * 1000

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200 μL of stock

μL of 10nM stock: 200
μL DNA: 2000 / nM DNA
μL EB: μL of 10nM stock - μL DNA

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custom volume of stock

μL of 10nM stock: custom
μL DNA: (μL of 10nM stock * 10) / nM DNA
μL EB: ul of 10nM stock - μL DNA