Gaius-Augustus / GALBA

GALBA is a pipeline for fully automated prediction of protein coding gene structures with AUGUSTUS in novel eukaryotic genomes for the scenario where high quality proteins from one or several closely related species are available.
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Change working directory? #36

Closed kushalsuryamohan closed 1 year ago

kushalsuryamohan commented 1 year ago

Hi, I've installed GALBA through singularity but I'm unable to change the working directory. It currently defaults to my home directory which has a limited disk quota.

I've tried the following: singularity exec galba.sif galba.pl --species=Botasp --genome=BotAsp_cleaned_renamed.fasta --prot_seq=uniprot_sprot.fasta --threads=8 --skipGetAnnoFromFasta --workingdir=/SCRATCH/GENOME_ANALYSIS/Botasp

however, here's the error I see:

Use of uninitialized value $workDir in -d at /opt/GALBA/scripts/galba.pl line 496. Use of uninitialized value $workDir in concatenation (.) or string at /opt/GALBA/scripts/galba.pl line 497. Use of uninitialized value $workDir in mkdir at /opt/GALBA/scripts/galba.pl line 499. Use of uninitialized value $workDir in concatenation (.) or string at /opt/GALBA/scripts/galba.pl line 499. ERROR: in file /opt/GALBA/scripts/galba.pl at line 500 Failed to create directory !

Can you please help me resolve this?