Generade-nl / TULIP

TULIP - The Uncorrected Long read Itegration Pipeline
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Multiple errors in tulipbulb.perl run #10

Open milw opened 6 years ago

milw commented 6 years ago

Hi, I've run bwa mem and tulipseed and generated what looks like appropriate data, but when I try to run tulipbulb I'm getting perl errors for every bundling step it appears- this is repeated over and over:

Use of uninitialized value $headerin in scalar chomp at tulipbulb.perl line 490, line 16008. Use of uninitialized value $seqin in scalar chomp at tulipbulb.perl line 491, line 16008. Use of uninitialized value $headerin in substitution (s///) at tulipbulb.perl line 492, line 16008. Use of uninitialized value $headerin in substitution (s///) at tulipbulb.perl line 493, line 16008. Use of uninitialized value $headerin in string eq at tulipbulb.perl line 494, line 16008. Use of uninitialized value $headerin in hash element at tulipbulb.perl line 497, line 16008. Use of uninitialized value $headerin in hash element at tulipbulb.perl line 499, line 16008.

final stdout is

Use of uninitialized value within %seedsequence in uc at tulipbulb.perl line 333, line 92512. print() on closed filehandle OUTPUT at tulipbulb.perl line 340, line 92512. print() on closed filehandle OUTPUT at tulipbulb.perl line 341, line 92512. 48563 nt written to 367 scaffolds Run ended print() on closed filehandle LOG at tulipbulb.perl line 177, line 92512.

and no output is generated. I've got Perl 5.22 if that's any help?

milw commented 6 years ago

Just realized my seeds are not named correctly:

M02484:23:000000000-AAB4V:1:1101:18766:2309 1:N:0:3 AATAGGTTAGCGGGACAAGGAAGAAAGTGCGATTGTATTAGTGTTCCGGGAAGAAAGGGAATCCTGCGCT TCTCTAAGAAAAGAAAAAGAGAGAGTTTACACAAGTAAAGTACTAAACGAGAAAAAAAGCATTTATCGAG AAAAGGTCCCATAGTCATATTTATATATATAGACTCCTCATATTTATATATATAATAGAAAGATGAAAGG TTTCTTTTTGTTCGGTCAGTCTGTTTCCTCTCGATTCACCTAAAACTTCTTTTGCCACTGACGTGATACA ATGAGCCATCTCATTCTACATTGAGTTAGCATCCTCACCTAACTTCCACTCCGTCTCATTAATTATTTTC TCTTTAAACATAACTTGCTTATCCCCTTTCCAATTCCACCACCTAATCCT