invalid feature coordinates (end<start!) at line

GenomeRIK / tama

Transcriptome Annotation by Modular Algorithms (for long read RNA sequencing data)

GNU General Public License v3.0

125 stars 24 forks source link

invalid feature coordinates (end<start!) at line #131

Open tky199996 opened 6 months ago

tky199996 commented 6 months ago

Hello, after I converted the bed file to a gtf file, I planned to convert the gtf file to fa, but the following error was displayed. How should I solve it? thank you very much for your help ![Uploading 1.png…]()

tky199996 commented 6 months ago

First, I used pbmm2 to align the lfnc sequence to the reference genome sequence and generate a bam file. Then I converted the bam file into a sam file, using the samtools tool for conversion, then using the tama_collapse.py command, and then using tama_merge.py command, but subsequently when I continue to use the SQANTI3 command, it prompts me the following, how can I solve it, does this have any impact?