In ancient DNA studies, a popular field is to recover ancient pathogen genomes (e.g. Yersinia pestis), i.e. the genomes of dead organisms where much of the cellular biomass has disintegrated. However the degraded DNA of the microbial cells can still be preserved (e.g. bound to skeletal mineral) and (majority) genome reconstruction is possible .
There is a debate in MInAS where such a sample/sequence fit into the MIxS schema. My initial reaction was that it would go into MIGSBacteria (as it's a single genome, not a whole metagenome).
Problem
As ancient DNA is very short, de novo methods do not work, and thus many researchers currently opt for short-read reference based mapping of the short reads to recover a genome-length conensus sequence.
aDNA uses reference and consensus based genome reconstruction, whereas MIGSBacteria has fields for assembly_software, but it is unclear what the definition is of assembly: is that purely de novo, or are reference based mappings allowed?
Possible solutions
Update the assembly_software term definition to specify this can be any form of assembly
Add an additional (presumably) enumerated term called assembly_method (suggested by @only1chunts ) that additionally specifies whether the assembly was denovo or reference-based
Context:
In ancient DNA studies, a popular field is to recover ancient pathogen genomes (e.g. Yersinia pestis), i.e. the genomes of dead organisms where much of the cellular biomass has disintegrated. However the degraded DNA of the microbial cells can still be preserved (e.g. bound to skeletal mineral) and (majority) genome reconstruction is possible .
There is a debate in MInAS where such a sample/sequence fit into the MIxS schema. My initial reaction was that it would go into MIGSBacteria (as it's a single genome, not a whole metagenome).
Problem
As ancient DNA is very short, de novo methods do not work, and thus many researchers currently opt for short-read reference based mapping of the short reads to recover a genome-length conensus sequence.
aDNA uses reference and consensus based genome reconstruction, whereas MIGSBacteria has fields for
assembly_software
, but it is unclear what the definition is of assembly: is that purely de novo, or are reference based mappings allowed?Possible solutions
assembly_software
term definition to specify this can be any form of assemblyassembly_method
(suggested by @only1chunts ) that additionally specifies whether the assembly wasdenovo
orreference-based