Whether tsRNAsearch is suitable for paired-end RNA-seq data？

[WZo0o]

Hello GiantSpaceRobot tsRNAsearch is a very amazing tool for tRNA mapping, but whether tsRNAsearch is suitable for paired-end RNA-seq data ?

[GiantSpaceRobot]

Hi there, It can work with paired-end data using a work-around. You can just cat the pair files together:

cat file1_1.fastq.gz file1_2.fastq.gz > file1.fastq.gz
cat file2_1.fastq.gz file2_2.fastq.gz > file2.fastq.gz
cat file3_1.fastq.gz file3_2.fastq.gz > file3.fastq.gz

You can then treat these new files as single-end as use the pipeline as normal.

[WZo0o]

Thanks for your solutions, I will try it.